CS0410

Sigma-Aldrich

Glutathione-S-Transferase (GST) Assay Kit

sufficient for 500 multiwell tests

EC Number:
NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 500 multiwell tests

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... GSTA4(2941), GSTM1(2944)

General description

The Glutathione S-Transferase (GST) Assay Kit is designed to measure total GSTactivity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma
and erythrocyte lysates

Application

The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.

Biochem/physiol Actions

Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.

Kit Components Only

Product No.
Description

  • Dulbecco's Phosphate Buffered Saline 100 mL

  • L-Glutathione Reduced 1 g

  • GST (Control) .1 mL

  • Sample Buffer 5 mL

  • Substrate (CDNB) 1.2 mL

Signal Word

Danger

Hazard Codes

T,N

Risk Statement

23/24/25-33-38-41-43-50/53

Safety Statement

24-28-36/37-45-60-61

RIDADR

UN 3316 9

WGK Germany

WGK 2

Flash Point(F)

381.2 °F - closed cup

Flash Point(C)

194 °C - closed cup

Certificate of Analysis
Certificate of Origin
Maria Sehlstedt et al.
Particle and fibre toxicology, 7, 21-21 (2010-08-24)
Biomass combustion contributes to the production of ambient particulate matter (PM) in rural environments as well as urban settings, but relatively little is known about the health effects of these emissions. The aim of this study was therefore to characterize...
Shunying Yang et al.
BMC plant biology, 20(1), 143-143 (2020-04-09)
Overuse of nitrogen fertilizers is often a major practice to ensure sufficient nitrogen demand of high-yielding rice, leading to persistent NH4+ excess in the plant. However, this excessive portion of nitrogen nutrient does not correspond to further increase in grain...
Didier Techer et al.
Environmental toxicology, 32(1), 227-240 (2015-12-18)
Gallic and pelargonic acids are biologically derived substances receiving a growing interest as eco-friendly biocides with potential applications in freshwater system management. However, some data gaps remain to address their chronic ecotoxicity issue, particularly for fish. This work aimed at...
Aleksandra Kołota et al.
International journal of environmental research and public health, 16(13) (2019-07-03)
The World Health Organization (WHO) reported that alcohol consumption is a serious problem in adolescents. The aim of the study was to assess the influence of the time of exposure of various alcoholic beverages on body mass as well as...
Viviana Maresca et al.
International journal of molecular sciences, 21(18) (2020-09-10)
Oxidative damage (production and localization of reactive oxygen species) and related response mechanisms (activity of antioxidant enzymes), and induction of Heat Shock Protein 70 expression, have been studied in the toxi-tolerant liverwort Conocephalum conicum (Marchantiales) in response to cadmium stress...

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