Documents

CS0420

Sigma-Aldrich

phospho-Retinoblastoma (pThr821) ELISA human

phospho-Rb (pThr821), sufficient for 96 determinations

Synonym(s):
Retinoblastoma ELISA, Rb ELISA
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... RB1(5925)

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General description

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).

Application

Designed for the quantitative determination of human retinoblastoma (Rb).
phospho-Rb (pThr821) ELISA detects human retinoblastoma phosphorylated on threonine 821 in cell lysates.

Biochem/physiol Actions

Rb, the tumor suppressor product of the retinoblastoma susceptibility gene, is a 110 kDa protein that plays an important role in regulating cell growth and differentiation. The cell cycle inhibitory function is abrogated when Rb undergoes phosphorylation, catalyzed by the complex of cyclins/cyclin-dependent protein kinases (cdks).

Features and Benefits

The kit offer a fast (total assay time 4 hours), sensitive (sensitivity 0.1 ng/mL for non-phosphorylated and <0.8 units/mL for phosphorylated Rb) and easy alternative to immunoblotting or other bioassays. Precoated plates are provided in the kit, incubations proceed at room temperature and no proprietary equipment is required.

Analysis Note

The sensitivity is ~1 unit/mL of Rb (pThr821), which is 25 times more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.

Kit Components Only

Product No.
Description

  • Phospho-Retinoblastoma (pThr821) Standard 4 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal Anti-Retinoblastoma (Rb) Antibody-Coated 96 wells plate 1 ea

  • Anti-Phospho-Retinoblastoma (Rb) (pThr821) Antibody 11 mL

  • Anti-Rabbit IgG-HRP, Concentrate (100X) 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized Chromogen (TMB) 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive Strips 3 ea

See All (10)

Safety Statement

23-24/25

RIDADR

UN 1789 8/PG 3

Certificate of Analysis
Certificate of Origin

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