A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).
Designed for the quantitative determination of human retinoblastoma (Rb).
phospho-Rb (pThr821) ELISA detects human retinoblastoma phosphorylated on threonine 821 in cell lysates.
Rb, the tumor suppressor product of the retinoblastoma susceptibility gene, is a 110 kDa protein that plays an important role in regulating cell growth and differentiation. The cell cycle inhibitory function is abrogated when Rb undergoes phosphorylation, catalyzed by the complex of cyclins/cyclin-dependent protein kinases (cdks).
Features and Benefits
The kit offer a fast (total assay time 4 hours), sensitive (sensitivity 0.1 ng/mL for non-phosphorylated and <0.8 units/mL for phosphorylated Rb) and easy alternative to immunoblotting or other bioassays. Precoated plates are provided in the kit, incubations proceed at room temperature and no proprietary equipment is required.
The sensitivity is ~1 unit/mL of Rb (pThr821), which is 25 times more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.