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CS0430

Sigma-Aldrich

Tau ELISA mouse

sufficient for 96 determinations

Synonym(s):
Tau ELISA
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... MAPT(4137)

General description

A solid phase sandwich enzyme-linked-immunosorbent assay (ELISA).

Application

The assay is a solid phase sandwich enzyme linked-immunosorbent assay (ELISA) for the in vitro quantitative determination of mouse Tau in mouse brain homogenates, cell extracts, buffered solutions, or cell culture media. The assay will recognize both natural and recombinant mTau.
The assay uses a monoclonal capture antibody specific for mouse tau that has been coated onto multiwell strips. Standard dilutions and samples are incubated 2 hours at RT. Tau antigen binds to the capture antibody. After a wash, a detection antibody specific for the non-phosphorylated or phosphorylated protein is incubated 1 hr at RT, which results in binding to the immobilized Tau protein. An anti-rabbit IgG-HRP binds to the immobilized protein completing the four-member sandwich. The reaction is visualized by tetramethylbenzidine (TMB) substrate, followed by the stop solution. The intensity of the yellow color measured in a multiwell plate reader at 450 nm is directly proportional to the concentration of Tau in original sample. The unknown concentrations are calculated from the standard curve run with each assay

Features and Benefits

Tau ELISA is a simple, sensitive, and specific alternative to immunoblotting and RIA.

Analysis Note

The sensitivity is <15 pg/mL. Performance characteristics include linearity and recovery.

Kit Components Only

Product No.
Description

  • Mouse Tau Standard 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal Anti-mouse Tau Antibody-Coated 96-Well Plate, 1 ea

  • Anti-Tau Detection Antibody, from rabbit 11 mL

  • Anti-rabbit IGG- HRP, (100x) concentrate 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer Concentrate (25x); 100 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB) 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive Non Hazardous 3 ea

See All (10)

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
Chun-Yan Jian et al.
Molecular medicine reports, 16(6), 9457-9463 (2017-11-21)
Doxorubicin (DOX) has been widely used to treat cancers as a first‑line antitumor drug. However, it causes severe, irreversible, dose‑dependent cardiotoxicity. To evaluate the protective effects of naringin (NRG) on cardiotoxicity, the authors investigated the molecular mechanism of the p38MAPK...

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