Documents

CS0450

Sigma-Aldrich

Src ELISA human

sufficient for 96 determinations

Synonym(s):
Src ELISA
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Application

Src ELISA detects and quantifies the level of human Src protein independent of its phosphorylation state from lysates of cells. It can be used to normalize the Src content of the samples when examining quantities of phosphorylated sites on Src using Phospho-Src pTyr418 ELISA (CS0460).
Src ELISA is designed to detect and quantify human Src protein. c-Src (pp60c-src), the cellular homolog of the Rous sarcoma virus protein v-Src, is the protein product of the c-src proto-oncogene. Src localizes to the cytoplasm, plasma membrane, focal adhesions, and cadherin-containing cell junctions. Functions include cell adhesion and migration, enhancement of cell survival, and regulation of cell division.

Features and Benefits

This assay is a fast (total time 4 hours), sensitive, and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates, ready-to-use reagents, and incubation at room temperature.

Analysis Note

The sensitivity is <1 ng/mL, which is as sensitive as immunoblotting. No cross-reactivity is shown with p38, p53, and Akt. The assay has 100% cross-reactivity with Fyn, but not Lyn.

Kit Components Only

Product No.
Description

  • Src Standard 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-Src -Coated 96 wells plate 1 ea

  • Anti-Src Antibody 11 mL

  • Anti-Rabbit IgG-HRP, Concentrate (100X) 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized TMB Chromogen 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive Strips 3 ea

See All (10)

Hazard Codes

B

Safety Statement

23-24/25

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin

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