Src ELISA is designed to detect and quantify human Src protein. c-Src (pp60c-src), the cellular homolog of the Rous sarcoma virus protein v-Src, is the protein product of the c-src proto-oncogene. Src localizes to the cytoplasm, plasma membrane, focal adhesions, and cadherin-containing cell junctions. Functions include cell adhesion and migration, enhancement of cell survival, and regulation of cell division.
phospho-Src (pTyr418) ELISA detects and quantifies the level of human Src protein phosphorylated on tyrosine 418 from cell lysates. For normalizing Src content, use Src ELISA (CS0450), which is independent of phosphorylation state.
Features and Benefits
This assay is a fast (total time 4 hours), sensitive, and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates, ready-to-use reagents, and incubation at room temperature.
The sensitivity is <1 units/mL of Src (pTyr418), which is as sensitive as immunoblotting. The specificity was confirmed by peptide competition showing that only the phosphopeptide containing the phosphorylated tyrosine could block the ELISA signal.