Documents

CS0460

Sigma-Aldrich

phospho-Src (pTyr418) ELISA human

sufficient for 96 determinations

Synonym(s):
Src ELISA
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Application

Src ELISA is designed to detect and quantify human Src protein. c-Src (pp60c-src), the cellular homolog of the Rous sarcoma virus protein v-Src, is the protein product of the c-src proto-oncogene. Src localizes to the cytoplasm, plasma membrane, focal adhesions, and cadherin-containing cell junctions. Functions include cell adhesion and migration, enhancement of cell survival, and regulation of cell division.
phospho-Src (pTyr418) ELISA detects and quantifies the level of human Src protein phosphorylated on tyrosine 418 from cell lysates. For normalizing Src content, use Src ELISA (CS0450), which is independent of phosphorylation state.

Features and Benefits

This assay is a fast (total time 4 hours), sensitive, and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates, ready-to-use reagents, and incubation at room temperature.

Analysis Note

The sensitivity is <1 units/mL of Src (pTyr418), which is as sensitive as immunoblotting. The specificity was confirmed by peptide competition showing that only the phosphopeptide containing the phosphorylated tyrosine could block the ELISA signal.

Kit Components Only

Product No.
Description

  • Phospho-Src pTyr418 Standard 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-Src-Coated 96 well plate 1 ea

  • Anti-Phospho-Src pTyr418 11 mL

  • Anti-Rabbit IgG-HRP Concentrate (100X) 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB) 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive strips 3 ea

See All (10)

Safety Statement

23-24/25

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin

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