Documents

CS0480

Sigma-Aldrich

phospho-STAT1 (pTyr701) ELISA human

sufficient for 96 determinations

Synonym(s):
STAT1 ELISA
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... STAT1(6772)

General description

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).

Application

The ELISA is designed to detect and quantify the level of STAT1 phosphorylated on tyrosine 701 from lysates of human cells or tissues. It does not cross react with mouse or rat cells. For normalizing the STAT1 content of the samples, a STAT1 ELISA kit that is indepentent of phosphorylation status is available (CS0470).
The assay is designed to detect and quantify the level of STAT1.

Biochem/physiol Actions

STAT1, a member of the STAT family of proteins, controls the transcription of specific genes in response to cytokine stimulation. STAT1 mediates apoptotic signals from interferon γ stimulation and may modulate anti-apoptotic signaling arising from NF-κB.

Features and Benefits

STAT1 ELISA is a fast (total time 4 hours), sensitive, and specific alternative to immuniblotting and RIA.

Analysis Note

The sensitivity is <0.9 units/mL, which is 4x more than immunoblotting. The specificity was confirmed by peptide competition showing that only the phosphopeptide containing the phosphorylated tyrosine 701 blocks the ELISA signal.

Kit Components Only

Product No.
Description

  • Phospho-STAT1 (pTyr701) Standard 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Sample Treatment Buffer 10 mL

  • Monoclonal-Anti-STAT1-Coated 96 well plate 1 ea

  • Anti-Phospho-STAT1 (pTyr701) 11 mL

  • Anti-Rabbit IgG-HRP, Concentrate (100X) 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB) 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive Strips 3 ea

See All (11)

Safety Statement

23-24/25

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin

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