A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).
The ELISA is designed to detect and quantify the level of STAT1 phosphorylated on tyrosine 701 from lysates of human cells or tissues. It does not cross react with mouse or rat cells. For normalizing the STAT1 content of the samples, a STAT1 ELISA kit that is indepentent of phosphorylation status is available (CS0470).
The assay is designed to detect and quantify the level of STAT1.
STAT1, a member of the STAT family of proteins, controls the transcription of specific genes in response to cytokine stimulation. STAT1 mediates apoptotic signals from interferon γ stimulation and may modulate anti-apoptotic signaling arising from NF-κB.
Features and Benefits
STAT1 ELISA is a fast (total time 4 hours), sensitive, and specific alternative to immuniblotting and RIA.
The sensitivity is <0.9 units/mL, which is 4x more than immunoblotting. The specificity was confirmed by peptide competition showing that only the phosphopeptide containing the phosphorylated tyrosine 701 blocks the ELISA signal.