The kit is used for the measurement of the kinase activity, activation, and inhibition. Applications include: studying the kinase pathway, MEK activators and inhibitors, and identifying extracellular stress stimuli that are converted into specific cellular responses through the activation of the specific kinase signaling pathway.
MEK activity is assayed by immunodetection. The endogenous kinase of interest is immuonoprecipitated using the kinase specific antibody and EZview Red Protein A. The immunoprecipitated kinase phosphorylates a specific substrate and kinase activity is detected by immunoblotting the phosphorylated substrate using phospho-specific antibodies.