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CS0490

Sigma-Aldrich

MEK Activity Assay Kit

sufficient for 50 reactions

Pricing and availability is not currently available.

usage

sufficient for 50 reactions

shipped in

dry ice

storage temp.

−20°C

Application

The kit is used for the measurement of the kinase activity, activation, and inhibition. Applications include: studying the kinase pathway, MEK activators and inhibitors, and identifying extracellular stress stimuli that are converted into specific cellular responses through the activation of the specific kinase signaling pathway.
MEK activity is assayed by immunodetection. The endogenous kinase of interest is immuonoprecipitated using the kinase specific antibody and EZview Red Protein A. The immunoprecipitated kinase phosphorylates a specific substrate and kinase activity is detected by immunoblotting the phosphorylated substrate using phospho-specific antibodies.

Features and Benefits

  • A non-radioactive sensitive assay that can also be used with radioactivity if required (protocol provided).
  • Contains all the reagents for the immunoprecipitaion and phosphorylation reactions plus a detection antibody.
  • Easy, convenient, and user-friendly.
  • The EZview Red Affinity Gel allows a better visualization of the precipitated fraction.
  • No requirement for a secondary antibody, reducing background.
  • Contains a specific inhibitor for further verification of the kinase specificity.

Kit Components Only

Product No.
Description

  • Assay Buffer for Kinase Activity 1 mL

  • Anti-MAP Kinase Kinase .1 mL

  • MAP Kinase, Non-activated, from rat .1 mg

  • Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) .1 mL

  • U1026 (MEK Inhibitor) 1 mg

  • Wash Buffer 10× 8 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • P6486EzView Red Protein A 1 mL

Certificate of Analysis
Certificate of Origin

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