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CS0500

Sigma-Aldrich

Amyloid Precursor Protein ELISA human

Synonym(s):
APP ELISA
Pricing and availability is not currently available.

shipped in

wet ice

storage temp.

2-8°C

General description

It is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA) for the quantitative determination of APP protein in cell lysates. A monoclonal antibody specific for APP (regardless of phosphorylation state) has been coated onto the multiwell plate. APP standards, controls and unknown samples are incubated 2 hours at RT and APP antigen binds to the immobilized (capture) antibody. Anti-APP biotinylated detection antibody is added and incubated 1 hr at RT binding to the immobilized APP. After removal of excess detection antibody, Streptavidin-(HRP) is added. This binds to the detection antibody to complete the four-member sandwich. A substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of APP present in the original specimen and the O.D. measured at 450 nm are used to calculate the concentration of APP.

Features and Benefits

APP ELISA is a sensitive (<0.4 ng/mL and 8x more sensitive than immunoblotting) and specific (100 ng/mL and no cross-reactivity with Tau, α-synuclein, β-synuclein, Aβ1-40, and Aβ1-42) alternative to immunoblotting and RIA.

Analysis Note

APP ELISA is designed to detect and quantify both natural and recombinant human APP protein in CSF and cell lysates. The capture antibody binds to the N-terminus of human APP, and the detection antibody recognizes the N-terminus of amyloid β peptide. The assay detects the isoforms APP770, APP751 (soluble APP), APP733, and APP695. It also recognizes soluble APPα, but not soluble APPβ.

Kit Components Only

Product No.
Description

  • APP Standard 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-APP-Coated 96 well plate 1 ea

  • Monoclonal Anti-APP-Biotin 11 mL

  • Streptavidin-Peroxidase (HRP) Concentrate (100X) 1 vial

  • Streptavidin-HRP Diluent 25 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB) 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive strips 3 ea

See All (9)

Safety Statement

23-24/25

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
E-J Bae et al.
Cell death & disease, 6, e1901-e1901 (2015-10-09)
Lysosomal dysfunction has been implicated both pathologically and genetically in neurodegenerative disorders, such as Alzheimer's disease and Parkinson's disease (PD). Lysosomal gene deficiencies cause lysosomal storage disorders, many of which involve neurodegeneration. Heterozygous mutations of some of these genes, such...

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