CS0510

Sigma-Aldrich

TRAIL ELISA human

sufficient for 96 determinations

Synonym(s):
Tumor necrosis factor-related apoptosis-inducing ligand
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... TNFSF10(8743)

General description

TRAIL standard dilutions, controls and samples are added to wells precoated with a monoclonal antibody specific for TRAIL (regardless of phosphorylation state) . During the incubation, the TRAIL antigen binds to the immobilized (capture) antibody. An anti-human TRAIL biotin conjugate is added and binds to the immobilized TRAIL. Streptavidin-peroxidase (SAV-HRP) is added and acted upon by the bound enzyme to produce color. The intensity of color is directly proportional to the concentration of TRAIL present in the original specimen. The optical density measured at 450 nm in the multiwell plate reader is used to calculate the concentration of TRAIL.

Application

TRAIL ELISA is designed to detect and quantify the level of human TRAIL in serum, plasma, cell extracts, buffered solutions, or cell culture medium. TRAIL values for sera ranged from 100 to 1800 pg/mL, plasma (heparin) 300 to 1000 pg/mL, and commercially available pooled serum samples measured 100 to 500 pg/mL. A limited number of cell culture supernatants ranged from 0 to 200 pg/mL. Cell extracts of several human cell lines diluted 1:10 in standard diluent ranged from 0.45 to 15 ng/mL at 1 mg/mL of total protein.

Biochem/physiol Actions

TRAIL induces apoptosis in various tumor cell lines. TRAIL-mediated apoptosis occurs following its binding to DR4 or DR5 receptors. TRAIL expression is increased in transformed cell lines and various diseases including cancer and autoimmune disorders.

Analysis Note

The assay is specific for human TRAIL and does not cross-react with human DR4 (TRAIL receptor 1), DR5 (TRAIL receptor 2), basic FGF, IFN-γ, VEGF, TGF-α, TGF-β, FasL, mouse G-CSF, TNF-α, or bovine FGF.

Kit Components Only

Product No.
Description

  • TRAIL Standard, Lyophilized 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-Human TRAIL-Coated 96 well plate 1 ea

  • Anti-Human TRAIL Biotin-Conjugate 11 mL

  • Streptavidin-Peroxidase (SAV-HRP) Concentrate (100X) 1 vial

  • Streptavidin-HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB) 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive strips 3 ea

See All (10)

Hazard Codes

Xi

Risk Statement

36/37/38

Safety Statement

26-36

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
Y Kashii et al.
Journal of immunology (Baltimore, Md. : 1950), 163(10), 5358-5366 (1999-11-24)
Natural killer cells mediate spontaneously secretory/necrotic killing against rare leukemia cell lines and a nonsecretory/apoptotic killing against a large variety of tumor cell lines. The molecules involved in nonsecretory/apoptotic killing are largely undefined. In the present study, freshly isolated, nonactivated...

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