phospho-Bcl-2 (pSer70) ELISA human

sufficient for 96 determinations

Pricing and availability is not currently available.


sufficient for 96 determinations

shipped in

wet ice

storage temp.


Gene Information

human ... BCL2(596)

General description

The assay is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Standard dilutions and samples are added to the wells precoated with Anti-Bcl-2 antibody (regardless of phosphorylation state)and incubated 2 hours at RT. Bcl-2 binds to the capture antibody. A detection antibody specific for the non-phosphorylated or phosphorylated protein isadded and incubated 1 hr at RT, which results in binding to the immobilized IR protein. An anti-rabbit IgG-HRP binds to the immobilized protein completing the four-member sandwich. The reaction is visualized by tetramethylbenzidine (TMB) substrate, followed by the stop solution. The intensity of the yellow color, measured in a multiwell plate reader at 450 nm, is directly proportional to the concentration of Bcl-2 in standards and samples. The unknown concentrations are calculated from the standard curve run with each assay.


Bcl-2 ELISA is designed to detect and quantify the level of human Bcl-2 protein in cell lysates. Human Bcl-2, the mammalian homolog of the Caenorhabditis elegans protein CED-9, is a cellular oncoprotein with MW 26 kDa, which localizes to the mitochondrial outer membrane, the endoplasmic reticulum, and the nuclear membrane. Bcl-2 has two functions in the cell: to promote cell survival, and to encourage cell cycle exit and quiescence.
phospho-Bcl-2 (pSer70) ELISA detects and quantifies the level of human Bcl-2 protein, phosphorylated on serine 70. No cross-reactivity with mouse and rat cells was observed. For normalizing the content of phospho-Bcl-2 (pSer70) in the samples, use Sigma Bcl-2 (Total) ELISA, which is independent of phosphorylation status (Product Code CS0520).

Features and Benefits

Bcl-2 ELISA is a fast (total time 4 hours), sensitive, and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates and ready-to-use reagents.

Analysis Note

The analytical sensitivity is <0.5 units/mL, which is ~8x more than immunoblotting. Linear regression yielded a correlation coefficient of 0.99. The specificity showed that only the phosphopeptide containing phosphorylated serine 70 blocks the ELISA signal.

Kit Components Only

Product No.

  • Phospho-Bcl-2 pSer70 Standard, Lyophilized 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-Bcl-2 Coated 96 wells plate 1 ea

  • Anti-Phospho-Bcl-2 pSer70 Antibody 11 mL

  • Anti-Rabbit IgG-HRP, Concentrate (100X) 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive strips 3 ea

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