Documents

CS0570

Sigma-Aldrich

phospho-CREB (pSer133) ELISA

sufficient for 96 determinations

Synonym(s):
CREB ELISA, cAMP-Response Element-Binding protein
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... CREB1(1385)

Looking for similar products? Visit Product Comparison Guide

General description

The assay is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). Standard dilutions and samples are added to the wells precoated with capture antibody specific for CREB and incubated 2 hours at RT. CREB antigen binds to the capture antibody. A detection antibody specific for either non-phosphorylated or phosphorylated protein is incubated 1 hr at RT. An anti-rabbit IgG-HRP binds to the immobilized protein completing the four-member sandwich. The reaction is visualized by TMB substrate, followed by the stop solution. The intensity of the yellow color, measured in a multiwell plate reader at 450 nm, is directly proportional to the concentration of CREB in original sample. The unknown concentrations are calculated from the standard curve run with each assay.

Application

Detects and quantifies CREB phosphorylated on serine 133 in lysates of human and mouse cells. For normalizing the phospho-CREB (pSer133) content of the samples, a CREB ELISA independent of phosphorylation status is available from Sigma Product No. CS0580.

Biochem/physiol Actions

CREB, also referred to as cAMP-Response Element-Binding protein, activates transcription in response to stimuli that elevate cytoplasmic cAMP concentrations.

Features and Benefits

CREB ELISA is a fast (total time 4 hours), sensitive, and specific alternative to immunoblotting and RIA. The ease of use is facilitated by precoated plates and ready-to-use reagents.

Analysis Note

The analytical sensitivity is <0.09 units/mL. The specificity for phosphorylated CREB (pSer133) was confirmed by peptide competition.

Kit Components Only

Product No.
Description

  • Phospho-CREB pSer133 Standard, Lyophilized 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-CREB-Coated 96 well plate 1 ea

  • Anti-Phospho-CREB pSer133, 11 mL

  • Anti-Rabbit IgG-HRP, Concentrate (100X) 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized Chromogen TMB 25 mL

  • Stop Solution 25 mL

  • Plate covers, adhesive strips 3 ea

See All (10)

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
J Xing et al.
Molecular and cellular biology, 18(4), 1946-1955 (1998-04-07)
The mechanisms by which growth factor-induced signals are propagated to the nucleus, leading to the activation of the transcription factor CREB, have been characterized. Nerve growth factor (NGF) was found to activate multiple signaling pathways that mediate the phosphorylation of...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.