Documents

CS0580

Sigma-Aldrich

CREB ELISA

sufficient for 96 determinations

Synonym(s):
cAMP-Response Element-Binding protein
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... CREB1(1385)

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General description

The assay is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). Standard dilutions and samples are added to the wells precoated with capture antibody specific for CREB and incubated 2 hours at RT. CREB antigen binds to the capture antibody. A detection antibody specific for either non-phosphorylated or phosphorylated protein is incubated 1 hr at RT. An anti-rabbit IgG-HRP binds to the immobilized protein completing the four-member sandwich. The reaction is visualized by TMB substrate, followed by the stop solution. The intensity of the yellow color, measured in a multiwell plate reader at 450 nm, is directly proportional to the concentration of CREB in original sample. The unknown concentrations are calculated from the standard curve run with each assay.

Application

CREB ELISA detects CREB in lysates of human and mouse cells and can be used to normalize the CREB content of the samples when examining quantities of phosphorylated sites on CREB using phospho-CREB pSer133 ELISA (Prod. No. CS0570).

Biochem/physiol Actions

CREB, also referred to as cAMP-Response Element-Binding protein, activates transcription in response to stimuli that elevate cytoplasmic cAMP concentrations.

Features and Benefits

CREB ELISA is a fast (total time 4 hours), sensitive, and specific alternative to immunoblotting and RIA. The ease of use is facilitated by precoated plates and ready-to-use reagents.

Analysis Note

Sensitivity is <0.025 ng/mL, which is ~4x more than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.

Kit Components Only

Product No.
Description

  • CREB Standard, Lyophilized 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-CREB-Coated 96 well plate 1 ea

  • Anti-CREB 11 mL

  • Anti-Rabbit IgG-HRP, Concentrate (100X 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized Chromogen (TMB) 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Plastic sheet 3 ea

See All (10)

Safety Statement

23-24/25

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin

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