phospho-c-Met (pTyr1230/pTyr1234/pTyr1235)

sufficient for 96 determinations

scatter factor (HGF/SF), Hepatocyte Growth Factor Receptor, c-Met ELISA
Pricing and availability is not currently available.


sufficient for 96 determinations

shipped in

wet ice

storage temp.


General description

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody specific for c-Met (regardless of phosphorylation state) has been coated onto the wells of the multiwell plate strips provided. c-Met standard dilutions, control specimens, and unknown samples are incubated for 2 hours at RT. c-Met antigen binds to the immobilized (capture) antibody. After a wash, a anti-c-Met detection antibody specific for phosphorylated or non-phosphorylated protein is incubated for 1 hour at RT, which results in binding to the immobilized c-Met protein. An anti-rabbit IgG-HRP binds to the detection antibody to complete the four-member sandwich. The rection is visualized by TMB substrate. The intensity of the yellow color is directly proportional to the concentration of c-Met present in the original specimen. The optical density measured at 450 nm in the multiwell plate reader is used to calculate the concentration of c-Met. The unknown concentrations are calculated from the standard curve run with each assay.


Designed to quantify full length human c-Met protein in cell lysates. The assay does not detect soluble c-Met.
phospho-c-Met (pTyr1230/pTyr1234/pTyr1235) detects c-Met protein phosphorylated on tyrosines 1230, 1234, and 1235. The sensitivity is <0.25 units/mL, which is twice as sensitive as immunoblotting.

Biochem/physiol Actions

c-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor (also known as scatter factor). Many types of cancers exhibit sustained c-Met stimulation, overexpression, or mutation.

Features and Benefits

c-Met ELISA is a fast (total time 3.5 hours), sensitive (minimum detectable concentration >0.4 ng/ml) and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates.

Kit Components Only

Product No.

  • c-Met pTyr1230/pTyr1234/pTyr1235 Standard, Lyophilized 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-c-Met -Coated 96-Well Plate 1 ea

  • Anti-c-Met pTyr1230/pTyr1234/pTyr1235 Detection Antibody, from rabbit 11 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB) 25 mL

  • Anti-Rabbit IGG-HRP, (100x) concentrate 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer Concentrate (25x) 100 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive Non Hazardous 3 ea

See All (10)


UN 3316 9/PG 3

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