Documents

CS0590

Sigma-Aldrich

phospho-c-Met (pTyr1230/pTyr1234/pTyr1235)

sufficient for 96 determinations

Synonym(s):
scatter factor (HGF/SF), Hepatocyte Growth Factor Receptor, c-Met ELISA
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

General description

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody specific for c-Met (regardless of phosphorylation state) has been coated onto the wells of the multiwell plate strips provided. c-Met standard dilutions, control specimens, and unknown samples are incubated for 2 hours at RT. c-Met antigen binds to the immobilized (capture) antibody. After a wash, a anti-c-Met detection antibody specific for phosphorylated or non-phosphorylated protein is incubated for 1 hour at RT, which results in binding to the immobilized c-Met protein. An anti-rabbit IgG-HRP binds to the detection antibody to complete the four-member sandwich. The rection is visualized by TMB substrate. The intensity of the yellow color is directly proportional to the concentration of c-Met present in the original specimen. The optical density measured at 450 nm in the multiwell plate reader is used to calculate the concentration of c-Met. The unknown concentrations are calculated from the standard curve run with each assay.

Application

Designed to quantify full length human c-Met protein in cell lysates. The assay does not detect soluble c-Met.
phospho-c-Met (pTyr1230/pTyr1234/pTyr1235) detects c-Met protein phosphorylated on tyrosines 1230, 1234, and 1235. The sensitivity is <0.25 units/mL, which is twice as sensitive as immunoblotting.

Biochem/physiol Actions

c-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor (also known as scatter factor). Many types of cancers exhibit sustained c-Met stimulation, overexpression, or mutation.

Features and Benefits

c-Met ELISA is a fast (total time 3.5 hours), sensitive (minimum detectable concentration >0.4 ng/ml) and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates.

Kit Components Only

Product No.
Description

  • c-Met pTyr1230/pTyr1234/pTyr1235 Standard, Lyophilized 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-c-Met -Coated 96-Well Plate 1 ea

  • Anti-c-Met pTyr1230/pTyr1234/pTyr1235 Detection Antibody, from rabbit 11 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB) 25 mL

  • Anti-Rabbit IGG-HRP, (100x) concentrate 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer Concentrate (25x) 100 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive Non Hazardous 3 ea

See All (10)

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.