Documents

CS0640

Sigma-Aldrich

Heat Shock Protein 27 ELISA human

sufficient for 96 determinations

Synonym(s):
HSP27, Heat Shock Protein 27 ELISA
MDL number:
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... HSPB1(3315)

General description

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA). Standard dilutions and samples are added to the wells precoated with Anti-HSP27 capture antibody, specific for HSP27 regardless of phosphorylation state. After 2 hr at RT, a detection antibody specific for the non-phosphorylated or phosphorylated protein is added and incubated 1 hr at RT, which results in binding to the immobilized HSP27 protein. An anti-rabbit IgG-HRP binds to the immobilized protein completing the four-member sandwich. The reaction is visualized by TMB substrate, followed by the stop solution. The intensity of the yellow color, measured in a multiwell plate reader at 450 nm, is directly proportional to the concentration of HSP27 in original sample. The unknown concentrations are calculated from the standard curve run with each assay.

Application

HSP27 ELISA is designed to detect and quantify the level of HSP27, independent of its phosphorylation state. This assay is intended for the detection of HSP27 from lysates of human cells. It does not react with the mouse or rat HSP27 homolog, HSP25. It can be used to normalize the HSP27 content of the samples when examining quantities of phosphorylated sites on HSP27 using Sigma Phospho-Heat Shock Protein pSer82 ELISA (Product No. CS0630).

Biochem/physiol Actions

HSP27, a member of the ubiquitously expressed anti-apoptotic heat shock protein group, plays a role in maintaining cellular redox by increasing glutathione levels, stabilizing actin filaments, and blocking actin polymerization. It prevents the aggregation of improperly folded proteins. Recent evidence suggests that HSP27 protects cells from apoptosis by participation in several signaling pathways.

Analysis Note

The sensitivity is <0.3 ng/mL, which is 4 times more than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.

Kit Components Only

Product No.
Description

  • HSP27 Standard, Lyophilized 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-HSP27-Coated 96 well plate 1 ea

  • Anti-HSP27 6 mL

  • Anti-Rabbit IgG-HRP, Concentrate (100X 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized Chromogen, TMB 25 mL

  • Stop Solution 25 mL

  • Plate Cover, Adhesive Strips 3 ea

See All (10)

Safety Statement

23-24/25

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
P Pandey et al.
Oncogene, 19(16), 1975-1981 (2000-05-10)
The release of mitochondrial cytochrome c by genotoxic stress induces the formation of a cytosolic complex with Apaf-1 (mammalian CED4 homolog) and thereby the activation of procaspase-3 (cas-3) and procaspase-9 (cas-9). Here we demonstrate that heat-shock protein 27 (Hsp27) inhibits...

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