A solid phase sandwich enzyme-linked immunosorbent assay (ELISA). The optical density of standards measured at 450 nm in the multiwell plate reader is used to calculate IκBα.concentration in the original specimen.
This assay is designed to detect and quantify the level of IκBα protein phosphorylated on serine 32 in human cells. The kit is not recommended for detection of mouse and rat IκBa. For normalizing the IκBα content of the samples, IκBα ELISA, which is independent of phosphorylation status, is available from Sigma (Product No. CS0620).
IκBα (40 kDa), ubiquitously expressed among mammals, controls immune and inflammatory responses, cell division, and apoptosis. Numerous disease states including arthritis, asthma, and inflammatory bowel disease are associated with loss of IκBα regulation.
Features and Benefits
The assay is a sensitive and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates and ready-to-use reagents.
The sensitivity is <0.5 units/mL, which is 2x more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.