Documents

CS0660

Sigma-Aldrich

phospho-IκBα (pSer32) ELISA human

sufficient for 96 determinations

Synonym(s):
IκBα ELISA, Inhibitor of NFκBα
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... NFKBIA(4792)

General description

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA). The optical density of standards measured at 450 nm in the multiwell plate reader is used to calculate IκBα.concentration in the original specimen.

Application

This assay is designed to detect and quantify the level of IκBα protein phosphorylated on serine 32 in human cells. The kit is not recommended for detection of mouse and rat IκBa. For normalizing the IκBα content of the samples, IκBα ELISA, which is independent of phosphorylation status, is available from Sigma (Product No. CS0620).

Biochem/physiol Actions

IκBα (40 kDa), ubiquitously expressed among mammals, controls immune and inflammatory responses, cell division, and apoptosis. Numerous disease states including arthritis, asthma, and inflammatory bowel disease are associated with loss of IκBα regulation.

Features and Benefits

The assay is a sensitive and specific alternative to immunoblotting and RIA. The ease of run is facilitated by precoated plates and ready-to-use reagents.

Analysis Note

The sensitivity is <0.5 units/mL, which is 2x more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.

Kit Components Only

Product No.
Description

  • Phospho-IκBα pSer32 Standard, Lyophilized 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti- IκBα Coated 96 well plate 1 ea

  • Anti-Phospho-IκBα pSer32, 11 mL

  • Anti-Rabbit IgG-HRP, Concentrate (100X 1 vial

  • HRP Diluent 25 mL

  • Wash Buffer Concentrate, 25X 100 mL

  • Stabilized TMB Chromogen 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive Strips 3 ea

See All (10)

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin

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