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CS0670

Sigma-Aldrich

Osteocalcin ELISA human

sufficient for 96 determinations

Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... BGLAP(632)

Biochem/physiol Actions

Human Osteocalcin assay is a solid phase Enzyme Sensitivity Amplified Immunoassay (EASIA) performed on a multiwell plate. Heparin or EDTA plasma, and serum are the recommended samples. This method detects full length human Osteocalcin. N-terminal and C-terminal fragments have been tested and do not interfere. The assay uses a mixture of monoclonal antibodies (Mabs) directed against distinct epitopes of human osteocalcin. Standards and samples react with the capture monoclonal antibody (Mab 1) coated on the multiwell plate and with a monoclonal antibody (Mab 2) conjugated to horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated Mab 1 - human osteocalcin - Mab 2 - HRP, the multiwell plate is washed to remove unbound enzyme conjugated antibody. Bound enzyme conjugated antibody is measured through a chromogenic reaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the multiwell plate is then read at 450 nm wavelength. The amount of substrate turnover is determined colorimetrically by measuring the absorbance. The use of special reader (linearity up to 3 OD units) and a polychromatic data reduction method result in high sensitivity in the low range and in an extended standard range. The intensity of the color is directly proportional to the concentration of osteocalcin present in the original specimen.

Kit Components Only

Product No.
Description

  • Standard, 0 ng/mL, lyophilized 1 mL/vial

  • Monoclonal-Anti-Human Osteocalcin Antibody-Coated 96 wells plate 1 ea

  • Control 1, lyophilized, 1 mL/vial

  • Control 2, lyophilized 1 mL/vial

  • Monoclonal Anti-Human Osteocalcin-HRP Conjugate ( 1 mL/vial

  • Wash Buffer Concentrate (200X) 10 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL

  • Stop Solution 25 mL

  • Osteocalcin Standard 1, Lyophilized 1 mL/vial

  • Osteocalcin Standard 2, Lyophilized 1 mL/vial

  • Osteocalcin Standard 3, Lyophilized 1 mL/vial

  • Osteocalcin Standard 4, Lyophilized 1 mL/vial

  • Osteocalcin Standard 5, Lyophilized 1 mL/vial

See All (13)

Hazard Codes

B

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin

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