The assay is based on immunoprecipitation of the tyrosine kinase of interest using the specific anti-tyrosine kinase antibody. The immunoprecipitated kinase is incubated with its substrate (poly Glu, Tyr 4:1) in the presence of (γ32P)-ATP. The incorporation of the radioactive-phosphate is measured.
Protein tyrosine kinases (PTKs) are involved in regulation of cell to cell signaling concerning growth, differentiation, adhesion, motility, and death. They play significant roles in the development of many disease states, including diabetes and cancer.
The kit provides a peroxidase conjugated anti-phosphotyrosine antibody, which enables substrate analysis by dot blot. This assay serves as an alternative, short and easy non-radioactive detection method.