CS0730

Sigma-Aldrich

Tyrosine Kinase Assay Kit

sufficient for 50 reactions

Pricing and availability is not currently available.

usage

sufficient for 50 reactions

shipped in

dry ice

storage temp.

−20°C

General description

The assay is based on immunoprecipitation of the tyrosine kinase of interest using the specific anti-tyrosine kinase antibody. The immunoprecipitated kinase is incubated with its substrate (poly Glu, Tyr 4:1) in the presence of (γ32P)-ATP. The incorporation of the radioactive-phosphate is measured.

Biochem/physiol Actions

Protein tyrosine kinases (PTKs) are involved in regulation of cell to cell signaling concerning growth, differentiation, adhesion, motility, and death. They play significant roles in the development of many disease states, including diabetes and cancer.

Analysis Note

The kit provides a peroxidase conjugated anti-phosphotyrosine antibody, which enables substrate analysis by dot blot. This assay serves as an alternative, short and easy non-radioactive detection method.

Kit Components Only

Product No.
Description

  • Monoclonal Anti-Phosphotyrosine-Peroxidase antibody produced in mouse 1 vial

  • Wash Buffer 10X 8 mL

  • Assay Buffer For Tyrosine Kinase Activity 1 mL

  • EZviewTM Red Protein A Affinity Gel 1 mL

  • Epidermal Growth Factor Receptor 500 U

  • P81 Cellulose Phosphate Squares 10 ea

  • Mouse Monoclonal Anti-EGFR Antibody .05 mL

Hazard Codes

B

Certificate of Analysis
Certificate of Origin
Liana Adam et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(2), 641-651 (2018-10-26)
Little is known about the genetic alterations characteristic of small bowel adenocarcinoma (SBA). Our purpose was to identify targetable alterations and develop experimental models of this disease.Experimental Design: Whole-exome sequencing (WES) was completed on 17 SBA patient samples and targeted-exome...
Hang Zhou et al.
EMBO molecular medicine, 11(3) (2019-02-20)
Arthrogryposis is a group of phenotypically and genetically heterogeneous disorders characterized by congenital contractures of two or more parts of the body; the pathogenesis and the causative genes of arthrogryposis remain undetermined. We examined a four-generation arthrogryposis pedigree characterized by...

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