CS0740

Sigma-Aldrich

Acid phosphatase Assay Kit

 kit sufficient for 1,000 assays (multiwell plates),  kit sufficient for 100 assays (tubes)

eCl@ss:
42010102
NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

usage

 kit sufficient for 1,000 assays (multiwell plates)
 kit sufficient for 100 assays (tubes)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACP1(52), ACP2(53)

Application

The kit is used for the detection of acid phosphatase activity in tissues, whole cell extracts, column fractions and purified enzyme. The kit contains all the reagents required for a fast and simple acid phosphatase detection. The kit contains a standard solution and a control enzyme.

Biochem/physiol Actions

Acid phosphatase is one of the acid hydrolases that normally reside in lysosomes. It is a marker for the identification of lysosomes in sub-cellular fractionations.

Kit Components Only

Product No.
Description

  • p-Nitrophenyl phosphate tablets 5 mg 20 tablets

  • Citrate buffer solution, 0.09 M pH 4.8 100 mL

  • p-Nitrophenol Standard solution 10 mM 1 mL

  • Acid phosphatase control enzyme .2 mL

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Target Organs

Eyes

Hazard Codes

T

Risk Statement

23/24/25-39/23/24/25-40

Safety Statement

36/37-45

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Messika Revel et al.
Marine pollution bulletin, 150, 110627-110627 (2019-10-28)
The aim of the present study was to evaluate the presence and potential toxic effects of plastic fragments (<400 μm) of polyethylene and polypropylene on the Pacific oyster Crassostrea gigas. Oysters were exposed to environmentally relevant concentrations (0, 0.008, 10, 100 μg...
Anne M Taylor et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 166, 34-43 (2014-07-11)
The metalloid selenium is an essential element which at slightly elevated concentrations is toxic and mutagenic. In Australia the burning of coal for power generation releases selenium into estuarine environments where it accumulates in sediments. The relationship between selenium exposure...
Articles
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.
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