CY0100

Cytochrome c Reductase (NADPH) Assay Kit

 kit sufficient for 100 tests, determining cytochrome c reductase activity

NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

usage

 kit sufficient for 100 tests

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... POR(5447)

Biochem/physiol Actions

Eukaroytic NADPH-cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake.

Features and Benefits

  • Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation
  • Colorimetric measurement of NADPH-Cytochrome C Reductase activity in various sources after intoxication by drugs or challenge by xenobiotics
  • Analyze enrichment and function of ER

Analysis Note

The Cytochrome C Reductase Assay Kit contains all the reagents required for a fast and convenient determination of NADPH-Cytochrome C Reductase activity in cell and tissue extracts and in purified microsomes. It has been tested on samples prepared from various species of mammalian tissues such as liver, kidney, brain, spleen, and heart muscle, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition, it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae.

Kit Components Only

Product No.
Description

  • Cytochrome c 50 mg

Kit Components Also Available Separately

Product No.
Description
SDS

  • N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mg

  • C9363Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL

Hazard Codes

Xn,N

Risk Statement

20/21/22-51/53

Safety Statement

36/37-61

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Amanda Spring de Almeida et al.
Pharmacological research, 152, 104576-104576 (2019-12-04)
Breast carcinoma causes severe pain, which decreases the quality of life of patients. Current treatments produce adverse effects and have limited efficacy. Transient potential receptor ankyrin 1 (TRPA1) is related to the onset of cancer and neuropathic pain. The aim...
Camila Ritter et al.
Molecular neurobiology, 57(5), 2420-2435 (2020-02-26)
Central neuropathic pain is a common untreated symptom in progressive multiple sclerosis (PMS) and is associated with poor quality of life and interference with patients' daily activities. The neuroinflammation process and mitochondrial dysfunction in the PMS lesions generate reactive species....
Seasonal variations of pollution biomarkers in two populations of Corbicula fluminea (Muller).
Vidal, M.-L., et al.
Comparative Biochemistry and Physiology, 131, Part C, 133-151 (2002)
D Plonne et al.
Analytical biochemistry, 276(1), 88-96 (1999-12-10)
A novel method is described for the separation on a single gradient of the major intracellular organelles of the secretory pathway, the Golgi, the smooth endoplasmic reticulum, and the rough endoplasmic reticulum. Total microsomes were prepared from rat liver by...
Protein and gene structure and regulation of NADPH-Cytochrome P450 oxidoreductase.
Shen, A. L. and Kasper, C. B. et al.
Handbook of Experimental Pharmacology, 105, 35-59 (1993)
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