CYTOCOX1

Sigma-Aldrich

Cytochrome c Oxidase Assay Kit

sufficient for 100 tests, soluble and membrane bound mitochondria

NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 100 tests

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... COX1(4512)

Application

The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.

Biochem/physiol Actions

Cytochrome c oxidase EC 1.9.3.1 is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Features and Benefits

  • Simple, optimized protocol - Obtain reproducible results without special training needs
  • Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
  • Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
  • May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
  • Use to analyze the intactness of mitochondrial membranes
  • Simple colorimetric measurement of solution

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2506Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mg

  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mg

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
Uta Keil et al.
The Journal of biological chemistry, 279(48), 50310-50320 (2004-09-17)
Increasing evidence suggests an important role of mitochondrial dysfunction in the pathogenesis of Alzheimer's disease. Thus, we investigated the effects of acute and chronic exposure to increasing concentrations of amyloid beta (Abeta) on mitochondrial function and nitric oxide (NO) production...
Chao-Chin Liu et al.
Frontiers in microbiology, 11, 170-170 (2020-03-03)
Staphylococcus aureus spreads rapidly on the surface of soft agar medium. The spreading depends on the synthesis of biosurfactants, i.e., phenol soluble modulins (PSMs), which facilitate colony spreading of S. aureus. Our earlier study demonstrated that water accumulates in a...
Céline Aguer et al.
PloS one, 6(12), e28536-e28536 (2011-12-24)
Human primary myotubes are highly glycolytic when cultured in high glucose medium rendering it difficult to study mitochondrial dysfunction. Galactose is known to enhance mitochondrial metabolism and could be an excellent model to study mitochondrial dysfunction in human primary myotubes....
Filip J Larsen et al.
Cell metabolism, 13(2), 149-159 (2011-02-03)
Nitrate, an inorganic anion abundant in vegetables, is converted in vivo to bioactive nitrogen oxides including NO. We recently demonstrated that dietary nitrate reduces oxygen cost during physical exercise, but the mechanism remains unknown. In a double-blind crossover trial we...
Hikaru Miyagi et al.
The Journal of biological chemistry, 284(12), 7553-7560 (2009-01-23)
Cells of the yeast Saccharomyces cerevisiae contain three NAD kinases; namely, cytosolic Utr1p, cytosolic Yef1p, and mitochondrial Pos5p. Previously, the NADH kinase reaction catalyzed by Pos5p, rather than the NAD kinase reaction followed by the NADP(+)-dependent dehydrogenase reaction, had been...
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