D224

Sigma-Aldrich

4,5-Diaminofluorescein

5 mM in DMSO, ≥97% (HPLC)

Synonym(s):
DAF-2
MDL number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

assay

≥97% (HPLC)

availability

not available in Japan

concentration

5 mM in DMSO

storage temp.

2-8°C

Packaging

1 mg in glass bottle

Application

Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells.

Analysis Note

Final solution contains approx. 10% isopropanol by volume.

Legal Information

DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874,590

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Georgina A Rivera-Ingraham et al.
Frontiers in zoology, 13, 4-4 (2016-02-05)
Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize...
Julia Strahl et al.
PloS one, 15(5), e0232360-e0232360 (2020-05-08)
The free radical nitric oxide (NO) is a powerful metabolic regulator in vertebrates and invertebrates. At cellular concentrations in the nanomolar range, and simultaneously reduced internal oxygen partial pressures (pO2), NO completely inhibits cytochrome-c-oxidase (CytOx) activity and hence mitochondrial- and...
Isabella Echeverri et al.
American journal of human biology : the official journal of the Human Biology Council, 27(6), 822-831 (2015-05-07)
To evaluate the Relationship between maternal and newborn endothelial function and oxidative stress. Forty-three pregnant women and their offspring were evaluated. As markers of endothelial function, the flow-mediated dilation (FMD) was measured in pregnant women in the second and third...
Articles
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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