Sigma′s DirectLoad™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
DirectLoad™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.
Features and Benefits
- Popular band sizes
Sigma′s DirectLoad™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.
For optimal resolution, the recommended agarose gel concentration is 2.0%.
DirectLoad is a trademark of Sigma-Aldrich Co. LLC