D3812

Sigma-Aldrich

DirectLoad 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

NACRES:
NA.25
Pricing and availability is not currently available.

Quality Level

form

(liquid)

usage

sufficient for 100 loads

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s DirectLoad 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
DirectLoad 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Components

Sigma′s DirectLoad 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Cinzia Fabbro et al.
FEMS microbiology letters, 307(2), 158-164 (2010-04-24)
Culturable vibrios were isolated from seawater collected during an annual sampling study performed along the Gulf of Trieste coast (Northern Adriatic Sea), and conventional culturing and identification methods were used to investigate the presence of Vibrio parahaemolyticus. Biochemically selected Vibrio...
Solid-phase reverse transfection for intracellular delivery of functionally active proteins
Bulkescher R, et al.
Genome Research, 27(10), 1752-1758 (2017)
Articles
We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.
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Protocols
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
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JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
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