D6168

Sigma-Aldrich

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse

clone 1869A, ascites fluid

MDL number:

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1869A, monoclonal

contains

15 mM sodium azide

species reactivity

trout (Salmo gairdneri), sea urchin (Tripneustes gratilla), Strongylocentrotus purpuratus, Chlamydomonas

application(s)

western blot: 1:10,000 using Chlamydomonas reinhardtii flagella preparation

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

Specificity

Recognizes the intermediate chain (IC69, 69 kDa) of Chlamydomonas dynein, but does not recognize any other protein in the axoneme. Reacts strongly with the 76 kDa intermediate chain outer arm dynein of sea urchin (Tripneustes gratilla, Strongylocentrotus purpuratus) and with a single component (IC2, 73 kDa) of trout (Salmo gairdneri) outer arm dynein. Does not recognize any component in axonemes prepared from scallop gill cilia or sheep sperm, purified 14S and 22S dyneins from cilia of Tetrahymena thermophila, or chick brain extract.

Immunogen

outer arm dynein intermediate chain of Chlamydomonas.

Application

May be used for domain mapping within IC69, electron microscope studies on dynein particles, and for protein-protein association studies on purified outer arm dynein and axonemes from Chlamydomonas flagella.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

King, S.M., and Witman, G.B. et al.
Cell Movement: The Dynein ATPases, 1, 61-61 (1989)
S M King et al.
The Journal of biological chemistry, 266(13), 8401-8407 (1991-05-05)
We have used the zero-length cross-linker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) to examine protein-protein associations within purified outer arm dynein and axonemes from Chlamydomonas flagella. When axonemes were treated with 0.5-1 mM EDC in either the presence or absence of ATP/vanadate, a polypeptide...
S M King et al.
Cell motility and the cytoskeleton, 16(4), 266-278 (1990-01-01)
Outer-arm dynein purified from trout spermatozoa was disrupted by low-ionic-strength dialysis, and the resulting subunits were separated by sucrose density-gradient centrifugation. The intact 19 S dynein, containing the alpha- an beta-heavy chains, intermediate chains (ICs) 1-5 and light chains (LCs)...
Toshiyuki Oda et al.
Journal of cell science, 129(8), 1547-1551 (2016-03-05)
The docking complex is a molecular complex necessary for assembly of outer dynein arms (ODAs) on the axonemal doublet microtubules (DMTs) in cilia and flagella. The docking complex is hypothesized to be a 24-nm molecular ruler because ODAs align along...
D Marples et al.
The American journal of physiology, 274(2 Pt 2), F384-F394 (1998-03-05)
We investigated whether the motor protein cytoplasmic dynein and dynactin, a protein complex thought to link dynein with vesicles, are present in rat renal collecting ducts and associated with aquaporin-2 (AQP2)-bearing vesicles. Immunoblotting demonstrated cytoplasmic dynein heavy and intermediate chains...

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