D7295

Sigma-Aldrich

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

NACRES:
NA.52
Pricing and availability is not currently available.

Quality Level

form

liquid

concentration

10 mM

color

colorless

Featured Industry

Agriculture

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide.

Application

dNTP Mix has been used in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,etc. It has also been used in reverse transcription of total RNA to cDNA.
Deoxynucleotide Mix, 10mM has been used as a component of the DNA amplification mixture for polymerase chain reaction (PCR).
The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP and TTP sodium salts in high quality molecular biology grade water. One μL is sufficient for a standard 50 μL PCR reaction.

Suitable for routine and long PCR, manual and automated DNA sequencing, cDNA synthesis and labeling reactions.

Packaging

0.2, 20×0.2, 0.5 mL in poly bottle

Features and Benefits

  • Purity of each dNTP: Minimum 99%
  • Conveniently formulated; 1 μL is used per 50 μL PCR
  • Equimolar amounts of each dNTP means less pipetting
  • Minimize risk of contamination in PCR
  • UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the components to Product D7295, Deoxynucleotide Mix?

    The D7295 contains 10mM of each dATP, dCTP, dGTP and TTP in molecular biology grade water.  This is for a total concentration of 40 mM.

  6. Does Product No. D7295, Deoxynucleotide Mix contain dTTP or TTP?

    The product contains Thymidine 5´-triphosphate sodium salt.  Thymidine has a deoxy sugar, so it is a deoxythymidine.  

  7. How much Product D7295, Deoxynucleotide Mix, is needed for a PCR reaction?

    One μl is sufficient for a standard 50 μl PCR reaction.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Fiona S McDonnell et al.
Journal of glaucoma, 25(10), e834-e842 (2016-10-18)
Glaucoma is an optic neuropathy that affects 60 million people worldwide. There is an underlying fibrosis associated with the lamina cribrosa (LC) in glaucoma. DNA methylation is well established in regulating fibrosis and may be a therapeutic target for glaucoma....
Abril Sánchez-Botet et al.
Scientific reports, 8(1), 11797-11797 (2018-08-09)
Colorectal cancer (CRC) is one of the most common cancers worldwide, with 8-10% of these tumours presenting a BRAF (V600E) mutation. Cyclins are known oncogenes deregulated in many cancers, but the role of the new subfamily of atypical cyclins remains...
Unal Egeli et al.
Cancer investigation, 24(5), 484-491 (2006-08-31)
BRCA1 and BRCA2 gene mutations in patients with breast and/or ovarian cancer have been not characterized in the Turkish population until now. A total of 87 female subjects from two sets of families (38 families total) provided blood samples from...
Billions and billions sold: Pet-feeder crickets (Orthoptera: Gryllidae), commercial cricket farms, an epizootic densovirus, and government regulations make for a potential disaster
Weissman, et al.
Zootaxa, 3504(1), 67-88 (2012)
Shangmei Cao et al.
Journal of ethnopharmacology, 244, 112104-112104 (2019-08-09)
ShenYanXiaoBai granules is a traditional Chinese herbal medicine, It is used widely for the treatment of proteinuria caused by various kidney diseases. This study investigated the mechanism of Shenyan Xiaobai Granule in the treatment of nephritis proteinuria. 100 male wistar...
Articles
Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.
Read More
Protocols
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Read More
Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.
Read More
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Read More
Method for PCR amplification of Bacterial DNA with low contamination using MTP<sup>™</sup> <i>Taq</i> DNA Polymerase (<a href="#">D7442</a>)
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.