D8410

Sigma-Aldrich

Deoxyribonucleic acid, bacteriophage M13mp18 from Escherichia coli JM101

Single stranded (+ strand), buffered aqueous solution

Synonym(s):
M13mp18 phage DNA from Escherichia coli JM101
CAS Number:
MDL number:

grade

for molecular biology

form

buffered aqueous solution

mol wt

2.4 MDa (7,249 bases)

shipped in

dry ice

storage temp.

−20°C

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Application

M13 is a single-stranded DNA bacteriophage that infects male Escherichia coli strains. A chronic infection is set up during which the intracellular replicative form of the phage (RF-I, double-stranded DNA) serves as template for single-stranded ([+]strand) DNA production. The single-stranded DNA, packaged in phage coat proteins, is extruded through the host cell wall into the medium in large quantities.
M13mp18/19 are M13 derivatives. Each contains a multiple cloning site (MCS) within a portion of the gene for β-galactosidase. This DNA segment corrects a defect in β-galactosidase production by the host strain. The MCS regions of M13mp18/19 contain 13 unique restriction endonuclease sites, respectively.
Foreign DNA incorporated at the multiple cloning site results in loss of β-galactosidase production and consequently the inability to use lactose as a carbon source. Such lactose negative strains are detected as white plaques on medium containing X-Gal, a chromogenic substrate for β-galactosidase.
The intracellular, double-stranded (RF-I) form of the M13 phage can be manipulated like plasmid DNA, while the extracellular, single-stranded DNA is an excellent source of material for determining the sequence of insert DNA by the dideoxy procedure. The multiple cloning site of M13mp9/19 is in the opposite orientation relative to the cloning site of M13mp8/18.

Features and Benefits

  • DNA cloning vectors.
  • Single-stranded DNA serves as a sequencing template.

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA

Analysis Note

Unique sites: Within the β-galactosidase gene - Bgl I, Mst II, Pvu I; other unique sites - Aha II, ApaL I, Ava II, Bal I, Ban II, Bgl II, Bsm I, Dra III, HgiD I, Nae I, Nar I, Sau I, Sin I, SnaB I, Sno I.
Note: Attempts to clone foreign DNA at these "other" sites will likely lead to inactivation of essential phage functions.

Certificate of Analysis

Certificate of Origin

M Iu Mazina et al.
Tsitologiia, 55(4), 218-224 (2013-07-24)
DNA replication begins from multiple sites distributed thoughout the genome and named replication origins. Despite the increasing amount of data on the properties of replication origins, it is still unknown what factors(s) is the primary determinant of ORC localization. Su(Hw)...
Jong Bum Lee et al.
Journal of biomedical nanotechnology, 9(7), 1245-1249 (2013-08-06)
Recently, DNA has been used to guide the self-assembly of functional materials. Based on programmability of DNA, branched DNA nanostructures were created and precisely labeled with quantum dots and gold nanoparticles. The precise molecular recognition of DNA allows the precise...
Christine Keyser et al.
Medecine sciences : M/S, 29(6-7), 637-641 (2013-07-19)
The authors highlight the opportunities to reconstruct the human Eurasian steppe migration movements with the analyses of nuclear DNA markers (short tandem repeats on autosomal DNA and on the Y chromosome) as well as mitochondrial DNA markers. They studied 26 ancient...
Data disclosure crucial after DNA patent verdict.
Alex de Costa
Science (New York, N.Y.), 341(6149), 959-959 (2013-08-31)
Chao Liang et al.
Journal of nanoscience and nanotechnology, 13(6), 3999-4005 (2013-07-19)
LAMP is an isothermal amplification method that can achieve ultra-high sensitivity and specificity. However, the conventional detection of LAMP amplicons can lead to cross-contamination due to the need to open the reaction tube which contains a large number of amplicons....

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