D9443

SAFC

Dulbecco′s Modified Eagle′s Medium - low glucose

liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling in cell culture applications

Synonym(s):
DME, DMEM
MDL number:
NACRES:
NA.75
Pricing and availability is not currently available.

Quality Level

description

With 1000mg/L D-glucose, L-glutamine, and sodium bicarbonate. Without arginine, leucine, lysine, sodium pyruvate, and phenol red

sterility

sterile-filtered

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

HEPES: no
L-glutamine: 0.584 g/L
sodium pyruvate: no
NaHCO3: 3.7 g/L
phenol red: no
glucose: 1.0 g/L

shipped in

ambient

storage temp.

2-8°C

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Reconstitution

Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Mahesh Saqcena et al.
PloS one, 8(8), e74157-e74157 (2013-08-27)
In multicellular organisms, cell division is regulated by growth factors (GFs). In the absence of GFs, cells exit the cell cycle at a site in G1 referred to as the restriction point (R) and enter a state of quiescence known...
CRISPR-Cas9-mediated knockout of GCN2 reveals a critical role in sensing amino acid deprivation in bovine mammary epithelial cells.
Edick, et al.
Journal of Dairy Science, 104, 1123-1135 (2021)
Sona Hubackova et al.
Molecular oncology, 13(7), 1467-1489 (2019-03-29)
Radiation and chemotherapy represent standard-of-care cancer treatments. However, most patients eventually experience tumour recurrence, treatment failure and metastatic dissemination with fatal consequences. To elucidate the molecular mechanisms of resistance to radio- and chemotherapy, we exposed human cancer cell lines (HeLa...
Deven Patel et al.
The Journal of biological chemistry, 292(3), 936-944 (2016-12-14)
Lipids are important nutrients that proliferating cells require to maintain energy homeostasis as well as to build plasma membranes for newly synthesized cells. Previously, we identified nutrient-sensing checkpoints that exist in the latter part of the G
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