E6906

Sigma-Aldrich

Anti-EZH2 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-ENX-1, Anti-Enhancer of zeste homolog 2 (Drosophila)
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 100 kDa

species reactivity

human

application(s)

indirect immunofluorescence: 1:500-1:1,000 using HEK-293T cells fixed with paraformaldehyde-triton.
western blot: 1:500-1:1,000 using K562 cell lysates

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... EZH2(2146)

Immunogen

synthetic peptide corresponding to amino acids 477-493 of human EZH2, conjugated to KLH via a C-terminal added cysteine residue.

Application

Anti-EZH2 antibody produced in rabbit is suitable for indirect immunofluorescence at a dilution of 1:500-1:1,000 using HEK-293T cells fixed with paraformaldehyde-triton and for western blotting at a dilution of 1:500-1:1,000 using K562 cell lysates.

Biochem/physiol Actions

Histone-lysine N-methyltransferase EZH2 is an enzyme encoded by the EZH2 gene in humans. It belongs to the multiprotein polycomb repressive complex 2. Enhancer of zeste homologue 2 (EZH2) is crucially involved in epigenetic silencing by acting as a histone methyltransferase. It is overexpressed in many cancers and is involved in malignant cell proliferation and invasion. EZH2 may be associated with senescence and apoptosis in gastric cancer. It may act as a valuable biomarker for the diagnosis and prognosis of gastric cancer. EZH2 may have function in regulation of gene transcription and chromatin structure.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

H Chen et al.
Genomics, 38(1), 30-37 (1996-11-15)
To identify genes that map on human chromosome 21 (HC21) and that may contribute to the phenotype of Down syndrome (DS), exon trapping was applied to cosmid DNA from an HC21-specific library LL21NCO2-Q. More than 550 potential exons were cloned...
Warren Fiskus et al.
Molecular cancer therapeutics, 5(12), 3096-3104 (2006-12-19)
Human enhancer of zeste 2 (EZH2) protein belongs to the multiprotein polycomb repressive complex 2, which also includes suppressor of zeste 12 (SUZ12) and embryonic ectoderm development (EED). The polycomb repressive complex 2 complex possesses histone methyltransferase activity mediated by...
Lin Guo et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 35(7), 6649-6656 (2014-04-08)
The present meta-analysis aggregated the results of relevant studies to identify the role of zeste homolog 2 (EZH2) expression in gastric carcinogenesis among Asians. Related articles were found by searching the following electronic databases without language restrictions: PubMed, SpringerLink, Karger...
Jiang Wang et al.
Molecular medicine reports, 11(5), 3585-3592 (2015-01-13)
Renal cell carcinoma (RCC) has a high potential for bone metastasis; however, the molecular mechanisms underlying this metastasis have remained to be elucidated. The present study aimed to explore the expression levels of enhancer of zeste homolog 2 (EZH2), matrix...
J Bai et al.
Cell proliferation, 47(3), 211-218 (2014-04-18)
Enhancer of zeste homologue 2 (EZH2) is crucially involved in epigenetic silencing by acting as a histone methyltransferase. Although EZH2 is overexpressed in many cancers and is involved in malignant cell proliferation and invasion, the role of EZH2 in senescence...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service