E8033

Sigma-Aldrich

pFLAG-MAC Expression Vector

Bacterial vector for cytoplasmic expression of N-terminal Met-FLAG fusion proteins

Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous solution

conjugate

FLAG® tagged

shipped in

dry ice

storage temp.

−20°C

General description

The pFLAG-MAC Expression Vector is a 5.0 kb E. coli expression vector used for cloning and cytoplasmic expression of properly inserted open reading frame as an N-terminal Met-FLAG® fusion protein. The Met-FLAG fusion protein may be detected using Monoclonal ANTI-FLAG M2 (F3165), Monoclonal ANTI-FLAG M5 (F4042), and can be purified using the ANTI-FLAG M2 Affinity Gel (A2220).

The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein. The FLAG-BAP fusion protein may be detected using Monoclonal ANTI-FLAG M1 (F3040), or Monoclonal ANTI-FLAG M2 (F3165), or purified using ANTI-FLAG M1 Agarose Affinity Gel (A4596), or ANTI-FLAG M2 Affinity Gel (A2220).

Vector Maps and Sequences

Components

  • pFLAG-MAC Expression Vector 10 μg (E5644) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • pFLAG-ATS-BAP Control Plasmid 1 μg (C9079) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC
pFLAG-MAC is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

H Komatsuzawa et al.
The Journal of antimicrobial chemotherapy, 45(4), 421-431 (2000-07-15)
A Tn551 insert in a gene termed fmtB was shown to reduce oxacillin as well as Triton X-100 resistance in highly methicillin-resistant Staphylococcus aureus (MRSA) COL. Backcrosses of fmtB::Tn551 into S. aureus COL and into two genetically distinct MRSA strains...
Genome-wide identification and expression analysis of the GSK gene family in Solanum tuberosum L. under abiotic stress and phytohormone treatments and functional characterization of StSK21 involvement in salt stress.
Huang, et al.
Gene, 766, 145156-145156 (2020)
Vikramjit S Bajwa et al.
Plant physiology, 163(1), 30-42 (2013-07-12)
Brassinosteroids (BRs) are plant hormones that are perceived at the cell surface by a membrane-bound receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1). BRI1 interacts with BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1) to initiate a signal transduction pathway in which autophosphorylation and transphosphorylation of BRI1...
Julia A Ross et al.
Journal of bacteriology, 193(9), 2276-2289 (2011-03-02)
YscD is an essential component of the plasmid pCD1-encoded type III secretion system (T3SS) of Yersinia pestis. YscD has a single transmembrane (TM) domain that connects a small N-terminal cytoplasmic region (residues 1 to 121) to a larger periplasmic region...
Sandra Lobo et al.
The Journal of biological chemistry, 277(43), 41268-41273 (2002-08-24)
Most Ras proteins are posttranslationally modified by a palmitoyl lipid moiety through a thioester linkage. However, the mechanism by which this occurs is not known. Here, evidence is presented that the Ras2 protein of Saccharomyces cerevisiae is palmitoylated by a...

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