E8158

Sigma-Aldrich

pFLAG-ATS Expression Vector

Bacterial vector for periplasmic expression of N-terminal FLAG fusion proteins

grade

for molecular biology

form

buffered aqueous solution

conjugate

FLAG® tagged

shipped in

dry ice

storage temp.

−20°C

General description

The pFLAG-ATS Expression Vector is a 5.4 kb E. coli expression vector used for cloning and periplasmic expression of properly inserted open reading frame as an N-terminal FLAG fusion protein. The N-terminal FLAG® fusion protein may be detected using Monoclonal ANTI-FLAG M1, Catalog Number F3040, or Monoclonal ANTI-FLAG M2, Catalog Number F3165, or purified using ANTI-FLAG M1 Agarose Affinity Gel, Catalog Number A4596 or ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein.

Vector Maps and Sequences

Components

  • pFLAG-ATS Expression Vector 10 μg ( E5769) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA
  • pFLAG-ATS-BAP Control Plasmid 1 μg (C9079) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC
pFLAG-ATS is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

Miaoying Tian et al.
BMC biochemistry, 6, 15-15 (2005-08-25)
Kazal-like serine protease inhibitors are defined by a conserved sequence motif. A typical Kazal domain contains six cysteine residues leading to three disulfide bonds with a 1-5/2-4/3-6 pattern. Most Kazal domains described so far belong to this class. However, a...
Miaoying Tian et al.
Plant physiology, 138(3), 1785-1793 (2005-06-28)
The plant apoplast forms a protease-rich environment in which proteases are integral components of the plant defense response. Plant pathogenic oomycetes, such as the potato (Solanum tuberosum) and tomato (Lycopersicon esculentum) pathogen Phytophthora infestans, secrete a diverse family of serine...
Birendra Singh et al.
Microbiology (Reading, England), 154(Pt 3), 797-809 (2008-03-04)
We describe an ATP-binding cassette (ABC) transporter in Pseudomonas putida KT2440 that mediates the uptake of glutamate and aspartate. The system (AatJMQP, for acidic amino acid transport) is encoded by an operon involving genes PP1071-PP1068. A deletion mutant with inactivated...
D M Stewart et al.
The Journal of clinical investigation, 97(11), 2627-2634 (1996-06-01)
The Wiskott-Aldrich syndrome (WAS) is an X-linked disorder characterized by thrombocytopenia, eczema, disorders in cell-mediated and humoral immunity, and a proclivity to lymphoproliferative disease. The gene responsible encodes a 53-kD proline-rich protein of unknown function (WASP). We produced a FLAG-WASP...
P J Brown et al.
FEBS letters, 497(1), 31-38 (2001-05-30)
Micronemes are specialised organelles, found in all apicomplexan parasites, which secrete molecules that are essential for parasite attachment to and invasion of host cells. Regions of several microneme proteins have sequence similarity to the Apple domains (A-domains) of blood coagulation...
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