Rachel L Olsen et al.
Veterinary microbiology, 164(1-2), 164-170 (2013-02-26)
The human pathogens enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC), as well as the mouse pathogen Citrobacter rodentium encode type III secretion system (T3SS) effector proteins to promote their survival in the infected host. The mechanisms of action and...
Construction of expression vectors to produce affinity-tagged proteins in Pseudomonas.
Robin Couch et al.
BioTechniques, 32(6), 1230-1230 (2002-06-21)
Veronika Kucharova et al.
PloS one, 8(6), e66429-e66429 (2013-07-11)
mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly used in protocols for analysis of mRNA stability....
Tsuyoshi Miki et al.
Journal of bacteriology, 191(22), 6843-6854 (2009-09-22)
The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded...
Johanna M Schwingel et al.
Glycobiology, 18(6), 447-455 (2008-03-14)
Moraxella catarrhalis express three predominant forms of lipooligosaccharide (LOS) molecules on the bacterial surface. These major glycolipids contain specific carbohydrate epitopes that distinguish each glycoform into serotype A, B, or C LOS. All three serotypes, however, share a common glucose...