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E8901

Sigma-Aldrich

Monoclonal Anti-E2F1 antibody produced in mouse

~0.5 mg/mL, clone KH20, purified immunoglobulin, buffered aqueous solution

MDL number:

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

KH20, monoclonal

form

buffered aqueous solution

mol wt

antigen 53 kDa (may vary in a recombinant preparation)

species reactivity

Xenopus, human, mouse, rat

concentration

~0.5 mg/mL

application(s)

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using transfected 293T cells expressing E2F1

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... E2F1(1869)
mouse ... E2f1(13555)
rat ... E2f1(399489)

Specificity

The epitope recognized by the antibody resides in the N-terminal region of human E2F1 (amino acids 1-89). May be used for immunoprecipitation (native and denatured), immunoblotting immunocytochemistry, and gel mobility shift assay.

Immunogen

recombinant human E2F1.

Application

Monoclonal Anti-E2F1 antibody produced in mouse is suitable for the following applications:
  • Immunoprecipitation
  • Microarray
  • Western blotting (1-2μg/mL using transfected 293T cells expressing E2F1)

Biochem/physiol Actions

Transcription factor E2F1 is a protein encoded by the E2F1 gene in humans and belongs to E2F family of transcription factors. It is located on chromosome 20q11 and plays a critical role in the transcription of certain genes required for cell cycle progression. E2F1 may facilitate identification of additional E2F family members and detection of E2F1 abnormalities in human tumors. It plays a crutial role in regulating cell cycle and other biological processes in cells. E2F-1 gene expression is higher in rectal cancer tissue compared to normal tissue.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

J Lukas et al.
Molecular and cellular biology, 16(3), 1047-1057 (1996-03-01)
The E2F family of transcription factors regulate genes, whose products are essential for progression through the mammalian cell cycle. The transcriptional activity of the E2Fs is inhibited through the specific binding of the retinoblastoma protein, pRB, and the pRB homologs...
Chao Su et al.
The Journal of biological chemistry, 290(11), 7208-7220 (2015-01-30)
Tudor staphylococcal nuclease (Tudor-SN) is a multifunctional protein implicated in a variety of cellular processes. In the present study, we identified Tudor-SN as a novel regulator in cell cycle. Tudor-SN was abundant in proliferating cells whereas barely expressed in terminally...
Lin-Hai Yan et al.
Journal of cellular biochemistry, 115(1), 34-41 (2013-09-17)
Transcription Factor E2F-1 plays a critical role in cell cycle regulation and other biological processes in cells. However whether or not it is involved in the multi-drug resistance (MDR) process of gastric cancer has not been fully elucidated yet. To...
Yang Yan et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 35(2), 1113-1122 (2013-09-17)
Due to the anatomical structure of the rectum, the treatment of rectal cancer remains challenging. Ad-E2F, an oncolytic adenovirus containing the E2F-1 promoter, can selectively replicate within and kill cancer cells derived from solid tumors. Thus, this virus provides a...
M L Kuo et al.
Cancer research, 58(10), 2245-2252 (1998-05-30)
Ribonucleotide reductase (RR), the rate-limiting enzyme in the de novo synthesis of deoxynucleotide triphosphates (dNTPs), is a potential target for cancer therapy. We characterized the response of RR in a human cervical carcinoma cell line, Caski, after damage by ionizing...

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