E9770

Sigma-Aldrich

Escort Transfection Reagent

Lipid formulation for nucleic acid transfections

MDL number:
Pricing and availability is not currently available.

grade

Molecular Biology
for molecular biology

form

liquid (aqueous solution)

usage

 kit sufficient for 33 transfections (10 cm dish)
 kit sufficient for 70 transfections (3.5 cm dish)

concentration

2 mg/mL

storage temp.

2-8°C

General description

Escort is a (1:1) liposome formulation of the cationic lipid DOTAP and the neutral lipid DOPE . This liposome-forming compound is used for transfection of nucleic acids into eukaryotic cells.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 15 μl Escort and 5 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort:

Bone marrow stromal cells (human)
CHO-K1
CHO (DHFR-)
COS-1
COS-7
CV-1
D. mel
H357
HEK293
HeLa
HepG2
HSC-3
LnCap
MCF-7
NIH3T3
Oral Squamous Cell Carcinoma (human)
PAE
Sf9

Features and Benefits

  • Suitable for stable and transient transfection
  • High efficiency for a wide variety of cell types
  • Low toxicity
  • Compatible with both serum and serum-free transfection protocols
  • Ideal for Sf9 insect cells

Components

Escort formulation:
1 mg/mL DOTAP
1 mg/mL DOPE
20 mM MES
150 mM NaCl (pH 6.2)

Caution

Do not freeze.

Principle

A stable complex is formed when DOTAP/DOPE is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Other Notes

The given protocol was developed for transient transfection of HeLa cells using pSV40-CAT plasmid as a reporter gene.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Y Perrie et al.
Biochimica et biophysica acta, 1475(2), 125-132 (2000-06-01)
Plasmid DNA pRc/CMV HBS (5.6 kb) (100 microg) encoding the S (small) region of hepatitis B surface antigen was incorporated by the dehydration-rehydration method into liposomes composed of 16 micromol egg phosphatidylcholine (PC), 8 micromol dioleoylphosphatidylcholine (DOPE) and 1, 2-diodeoyl-3-(trimethylammonium)propane...
Kerstin Tabatt et al.
Journal of controlled release : official journal of the Controlled Release Society, 97(2), 321-332 (2004-06-16)
Cationic solid lipid nanoparticles (SLN) for gene transfer are formulated using the same cationic lipids as for liposomal transfection agents. To investigate the differences and similarities in structure and performance between SLN and liposomes, a SLN preparation (S1), its counterpart...
S J Collis et al.
Nucleic acids research, 29(7), 1534-1538 (2001-03-27)
The strand transferase RAD51 is a component of the homologous recombination repair pathway. To examine the contribution of RAD51 to the genotoxic effects of ionising radiation, we have used a novel ribozyme strategy. A reporter gene vector was constructed so...
G P Vlasov et al.
Bioorganicheskaia khimiia, 31(2), 167-174 (2005-05-14)
We studied the possibility of optimizing the DNA transfection properties of carriers based on lysine dendrimers of the third and the fifth generation, including those containing a chloroacetyl or a lipophilic palmitoyl moiety at C-end. The use of lysosome-destroying antibiotic...
Jens C Wallmichrath et al.
Journal of cellular and molecular medicine, 13(8B), 2593-2601 (2008-12-11)
A novel therapeutic approach for the treatment of bone defects is gene therapy assisted bone tissue engineering using bone marrow stromal cells (hBMSC). The aim of this study was to investigate the influence of human epidermal growth factor (hEGF) on...

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