ERK 1&2 ELISA is designed to detect and quantify the levels of human, mouse or rat ERK 1&2 protein, independent of its phosphorylation state.The ELISA provides the normal values of total amount of ERK 1&2 present in the samples, which are simultaneously tested for ERK 1&2 dually phosphorylated at threonine 185 and tyrosine 187. ERK1 and ERK2 are serine/threonine kinases expressed broadly in normal tissues and various cell lines. They are activated through the phosphorylation of a threonine and a tyrosine residue and activate a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as regulation of the cytoskeleton.
ERK (Extracellular Signal Regulated Kinase), also known as MAPK (Mitogen-Activated Protein Kinase), has two isoforms. ERK1, also known as MAP kinase 1 or p44 MAP kinase, and ERK2, also known as MAP kinase 2 or p42 MAP kinase. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with mitogens, hormones, growth factors, cytokines and bioactive peptides. They are expressed broadly in normal tissues and various cell lines. Phosphorylation of ERK1&2 on a threonine and a tyrosine residues is required for enzyme activity of ERK1 and ERK2. Phosphorylation activates a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as regulation of the cytoskeleton.
Features and Benefits
ERK 1&2 ELISAs offer a fast (total time 4 hours), sensitive (<16 pg/mL for non-phosphorylated and <0.8 units/mL for phosphorylated ERK 1&2), and specific (measures ERK 1&2 with no cross-reactivity from JNK and p38) alternative to immunoblotting or other bioassays.
The sensitivity is <16 pg/mL, which is 4 times more sensitive than immunoblotting. The assay shows no cross-reactivity with JNK and p38 MAPK. Linear regression yielded a correlation coefficient of 0.99.