Documents

EK0100

Sigma-Aldrich

ERK 1&2 ELISA

sufficient for 96 tests

Synonym(s):
MAPK ELISA, Extracellular Signal Regulated Kinase
MDL number:
Pricing and availability is not currently available.

usage

sufficient for 96 tests

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... MAPK1(5594), MAPK3(5595)

Looking for similar products? Visit Product Comparison Guide

Application

ERK 1&2 ELISA is designed to detect and quantify the levels of human, mouse or rat ERK 1&2 protein, independent of its phosphory­lation state.The ELISA provides the normal values of total amount of ERK 1&2 present in the samples, which are simultaneously tested for ERK 1&2 dually phosphorylated at threonine 185 and tyrosine 187. ERK1 and ERK2 are serine/threonine kinases expressed broadly in normal tissues and various cell lines. They are activated through the phosphorylation of a threonine and a tyrosine residue and activate a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as regulation of the cytoskeleton.

Biochem/physiol Actions

ERK (Extracellular Signal Regulated Kinase), also known as MAPK (Mitogen-Activated Protein Kinase), has two isoforms. ERK1, also known as MAP kinase 1 or p44 MAP kinase, and ERK2, also known as MAP kinase 2 or p42 MAP kinase. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with mitogens, hormones, growth factors, cytokines and bioactive peptides. They are expressed broadly in normal tissues and various cell lines. Phosphorylation of ERK1&2 on a threonine and a tyrosine residues is required for enzyme activity of ERK1 and ERK2. Phosphorylation activates a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as regulation of the cytoskeleton.

Features and Benefits

ERK 1&2 ELISAs offer a fast (total time 4 hours), sensitive (<16 pg/mL for non-phosphorylated and <0.8 units/mL for phosphorylated ERK 1&2), and specific (measures ERK 1&2 with no cross-reactivity from JNK and p38) alternative to immunoblotting or other bioassays.

Analysis Note

The sensitivity is <16 pg/mL, which is 4 times more sensitive than immunoblotting. The assay shows no cross-reactivity with JNK and p38 MAPK. Linear regression yielded a correlation coefficient of 0.99.

Safety Statement

24/25

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
Making new connections: role of ERK/MAP kinase signaling in neuronal plasticity.
S Impey et al.
Neuron, 23(1), 11-14 (1999-07-13)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.