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F1304

Sigma-Aldrich

Monoclonal Anti-FKHRL1 (FOXO3a) antibody produced in mouse

clone FR1, purified immunoglobulin, buffered aqueous solution

MDL number:
Pricing and availability is not currently available.

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FR1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~75 kDa

species reactivity

human

application(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using cell extracts of COS7 cells expressing human FKHRL1

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FOXO3(2309)

Immunogen

synthetic peptide corresponding to amino acids 653-668 of human FKHRL1, conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-FKHRL1 (FOXO3a) antibody produced in mouse is suitable for the following applications:
  • Immunocytochemistry.
  • Indirect ELISA
  • Microarray
  • Western blotting at a concentration of 0.25-0.5μg/mL using cell extracts of COS7 cells expressing human FKHRL1
  • Immunohistochemistry.

Biochem/physiol Actions

Forkhead box O3 is a human protein encoded by the FOXO3 gene and is mapped to chromosomal regions 6q21. It belongs to Forkhead box-O (FoxO) family transcriptional factors and is found to be expressed in all human adult tissue. FoxO family controls the expression of many genes that are involved in a variety of cellular processes such as affecting tumorigenesis, metabolism, stem cell maintenance and lifespan. Its plays an important role in cell cycle regulation, apoptosis, oxidative stress and DNA repair. FOXO transcription factors have the ability to repress Cyclin D1 transcription and may alter Cyclin D1 activity. It is also found to be involved in melanogenesis regulation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

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Astrid Eijkelenboom et al.
Cell reports, 5(6), 1664-1678 (2013-12-24)
Forkhead box O (FOXO) transcription factors regulate diverse cellular processes, affecting tumorigenesis, metabolism, stem cell maintenance, and lifespan. We show that FOXO3 transcription regulation mainly proceeds through the most active subset of enhancers. In addition to the general distinction between...
Ummuhani Ozel Turkcu et al.
Gene, 536(1), 129-134 (2013-12-18)
Vitiligo is an acquired epidermal pigment loss of the skin. Oxidative stress is one of the major theories in the pathophysiology of vitiligo. FOXO3A is the forkhead members of the class O (FOXO) transcription factors, and plays an important role...
Juewon Kim et al.
The Journal of investigative dermatology, 134(5), 1378-1388 (2013-11-29)
Forkhead box-O (FoxO) family transcriptional factors control the expression of many genes involved in a variety of cellular processes. Melanogenesis is an oxidizing process; therefore, many antioxidants are used to inhibit melanin production. However, their mechanism of action is poorly...
Naoki Suzuki et al.
The Journal of clinical investigation, 117(9), 2468-2476 (2007-09-06)
Forkhead box O (Foxo) transcription factors induce muscle atrophy by upregulating the muscle-specific E3 ubiquitin ligases MuRF-1 and atrogin-1/MAFbx, but other than Akt, the upstream regulators of Foxos during muscle atrophy are largely unknown. To examine the involvement of the...
Oliver Dick et al.
The Journal of biological chemistry, 285(25), 19354-19361 (2010-04-21)
Synaptic activity and the generation of nuclear calcium signals promote neuronal survival through a transcription-dependent process that is not fully understood. Here we show that one mechanism of activity-induced acquired neuroprotection involves the Forkhead transcription factor, FoxO3a, which is known...

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