F2055

Sigma-Aldrich

Anti-hFBXW7 antibody , Mouse monoclonal

clone FB407, purified from hybridoma cell culture

Synonym(s):
Anti-SEL-10 (C. elegance), Anti-ARCHIPELAGO (Drosophila), Anti-FBW7, Anti-F-box and WD40 domain protein 7, Anti-Cdc4 (S. serevisia)
MDL number:
NACRES:
NA.41
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

FB407, monoclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

rat, human, mouse

concentration

~2 mg/mL

application(s)

immunocytochemistry: 10-20 μg/mL using human A431 or HeLa cells
indirect ELISA: suitable
western blot: 5-10 μg/mL using human A431 cell extracts

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FBXW7(55294)
mouse ... Fbxw7(50754)

General description

FBXW7 is a member of F-box protein family, which acts as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). The proteins have a bipartite structure. The shared F-box motif links F-box protein to Skp1 and the core complex, whereas divergent protein-protein interaction motifs selectively bind their cognate substrates. There are three FBXW7 isoforms α, β and γ that share 10 out of 11 exons but they differ in their subcellular localization as well as in substrate recognition activity.
Monoclonal Anti-hFBXW7 (mouse IgG2a isotype) is derived from the hybridoma FB407 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino-acids of human hFBXW7 isoforms α β and γ. FBXW7 is located on human chromosome 4q31.

Specificity

The antibody recognizes human, mouse, and rat hFBXW7.

Immunogen

synthetic peptide corresponding to amino-acids 238-250 of human hFBXW7 isoforms α, β, and γ.

Application

Anti-hFBXW7 antibody, Mouse monoclonal has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry

Biochem/physiol Actions

F-box proteins is associated with various signalling pathways, such as nutrient sensing in yeast,conserved developmental pathways in plants and animals. These proteins mediate recognition of phosphorylated targets including Cyclin E, Myc, c-Jun, and Notch, leading to their ubiquitination and degradation. Furthermore, it was found mutated in breast, endometrial, ovarian, and colon cancer, and finally results in genomic instability. Similar to the inactivation mode of other known tumor suppressors, the SV40 large T antigen binds FBXW7 without detectible effects on its stability, and thus acts as an inhibitor of FBXW7 activity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

The Fbxw7/hCdc4 tumor suppressor in human cancer
Tan YM, et al.
Cancer letters, 271(1), 1-12 (2008)
Markus Welcker et al.
Current biology : CB, 14(20), 1852-1857 (2004-10-23)
The human tumor suppressor Fbw7/hCdc4 functions as a phosphoepitope-specific substrate recognition component of SCF ubiquitin ligases that catalyzes the ubiquitination of cyclin E , Notch , c-Jun and c-Myc . Fbw7 loss in cancer may thus have profound effects on...
Harith Rajagopalan et al.
Nature, 428(6978), 77-81 (2004-03-06)
Aneuploidy, an abnormal chromosome number, has been recognized as a hallmark of human cancer for nearly a century; however, the mechanisms responsible for this abnormality have remained elusive. Here we report the identification of mutations in hCDC4 (also known as...
K L Craig et al.
Progress in biophysics and molecular biology, 72(3), 299-328 (1999-12-03)
The ubiquitin system of intracellular protein degradation controls the abundance of many critical regulatory proteins. Specificity in the ubiquitin system is determined largely at the level of substrate recognition, a step that is mediated by E3 ubiquitin ligases. Analysis of...
Margaret S Ho et al.
Journal of biomedical science, 13(2), 181-191 (2006-02-08)
The eukaryotic protein degradation pathway involves the ubiquitin (Ub) modification of substrates targeted for degradation by the 26S proteasome. The addition of Ub, a process called ubiquitination, is mediated by enzymes including the E3 Ub ligases which transfer the Ub...

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