F3930

Sigma-Aldrich

Anti-FMR1 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-FMRP, Anti-Fragile X Mental Retardation Protein
MDL number:
Pricing and availability is not currently available.

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~80 kDa

species reactivity

human, rat (predicted), mouse (predicted)

concentration

~1 mg/mL

application(s)

western blot: 0.5-1.0 μg/mL using lysates of HEK-293T cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FMR1(2332)
mouse ... Fmr1(14265)
rat ... Fmr1(24948)

General description

FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. FMR1, also known as FMRP, FRAXA and Fragile X mental retardation 1 protein, is localized to the cytoplasm with two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one RGG box. The protein is highly expressed in brain and testis.

Immunogen

synthetic peptide corresponding to amino acids 122-136 of human FMR1, conjugated to KLH. The corresponding sequence is highly conserved (1 amino acid substitution) in rat and mouse.

Application

Anti-FMR1 (N-terminal) antibody is suitable for western blot at a concentration of 0.5-1.0μg/mL, using lysates of HEK-293T cells.

Biochem/physiol Actions

The FMR1 protein can binds RNA in vitro including two KH domains and an RGG box, which further influences the association with polyribosomes in vivo. It also contains a 60S ribosomal subunit interaction domain and a protein-protein interaction domain which triggers homomer and heteromer formation with each family member. Defects in FMR1 gene activity cause Fragile X syndrome, a common inherited form of mental retardation.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
Carla E M Golden et al.
Cerebral cortex (New York, N.Y. : 1991), 29(5), 2228-2244 (2019-03-17)
Fragile X syndrome (FXS) is a neurodevelopmental disorder caused by mutations in the FMR1 gene. It is a leading monogenic cause of autism spectrum disorder and inherited intellectual disability and is often comorbid with attention deficits. Most FXS cases are...
L Wan et al.
Molecular and cellular biology, 20(22), 8536-8547 (2000-10-25)
Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is...
F Tamanini et al.
Human molecular genetics, 6(8), 1315-1322 (1997-08-01)
Lack of expression of the fragile X mental retardation protein (FMRP) results in mental retardation and macroorchidism, seen as the major pathological symptoms in fragile X patients. FMRP is a cytoplasmic RNA-binding protein which cosediments with the 60S ribosomal subunit....
Dan O Wang et al.
Frontiers in molecular biosciences, 4, 93-93 (2018-01-10)
RNA transport and regulated local translation play critically important roles in spatially restricting gene expression in neurons. Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain
Y Zhang et al.
The EMBO journal, 14(21), 5358-5366 (1995-11-01)
Fragile X Mental Retardation Syndrome is the most common form of hereditary mental retardation, and is caused by defects in the FMR1 gene. FMR1 is an RNA-binding protein and the syndrome results from lack of expression of FMR1 or expression...

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