SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets

tablet, To prepare 10 mL

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SIGMAFAST Fast Red TR/Naphthol AS-MX tablets has been used in fluorescent in situ hybridization for the analysis of gene expression in neurons and synapses.
Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.


Each tablet set dissolved in 10 mL of deionized water yields a ready-to-use buffered solution.

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SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC


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Target Organs

Respiratory system


UN1760 - class 8 - PG 2 - basic - EHS - Corrosive liquids, n.o.s., HI: all

WGK Germany


Calcium imaging of basal forebrain activity during innate and learned behaviors
Harrison TC, et al.
Frontiers in Neural Circuits, 10, 36-36 (2016)
Shinjae Chung et al.
Nature, 545(7655), 477-481 (2017-05-18)
In humans and other mammalian species, lesions in the preoptic area of the hypothalamus cause profound sleep impairment, indicating a crucial role of the preoptic area in sleep generation. However, the underlying circuit mechanism remains poorly understood. Electrophysiological recordings and...
Kevin T Beier et al.
Cell reports, 26(1), 159-167 (2019-01-04)
Viral-genetic tracing techniques have enabled mesoscale mapping of neuronal connectivity by teasing apart inputs to defined neuronal populations in regions with heterogeneous cell types. We previously observed input biases to output-defined ventral tegmental area dopamine (VTA-DA) neurons. Here, we further...
In Situ Hybridization Methods (2015)
Chenyan Ma et al.
Neuron, 103(2), 323-334 (2019-06-11)
A crucial step in understanding the sleep-control mechanism is to identify sleep neurons. Through systematic anatomical screening followed by functional testing, we identified two sleep-promoting neuronal populations along a thalamo-amygdala pathway, both expressing neurotensin (NTS). Rabies-mediated monosynaptic retrograde tracing identified...
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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