F4523

Sigma-Aldrich

SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets

tablet, To prepare 10 mL

MDL number:
NACRES:
NA.83
Pricing and availability is not currently available.

Quality Level

form

tablet

storage temp.

−20°C

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Application

SIGMAFAST Fast Red TR/Naphthol AS-MX tablets has been used in fluorescent in situ hybridization for the analysis of gene expression in neurons and synapses.
Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.

Reconstitution

Each tablet set dissolved in 10 mL of deionized water yields a ready-to-use buffered solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Target Organs

Respiratory system

RIDADR

UN1760 - class 8 - PG 2 - basic - EHS - Corrosive liquids, n.o.s., HI: all

WGK Germany

WGK 3

Calcium imaging of basal forebrain activity during innate and learned behaviors
Harrison TC, et al.
Frontiers in Neural Circuits, 10, 36-36 (2016)
Shinjae Chung et al.
Nature, 545(7655), 477-481 (2017-05-18)
In humans and other mammalian species, lesions in the preoptic area of the hypothalamus cause profound sleep impairment, indicating a crucial role of the preoptic area in sleep generation. However, the underlying circuit mechanism remains poorly understood. Electrophysiological recordings and...
Kevin T Beier et al.
Cell reports, 26(1), 159-167 (2019-01-04)
Viral-genetic tracing techniques have enabled mesoscale mapping of neuronal connectivity by teasing apart inputs to defined neuronal populations in regions with heterogeneous cell types. We previously observed input biases to output-defined ventral tegmental area dopamine (VTA-DA) neurons. Here, we further...
The
In Situ Hybridization Methods (2015)
Chenyan Ma et al.
Neuron, 103(2), 323-334 (2019-06-11)
A crucial step in understanding the sleep-control mechanism is to identify sleep neurons. Through systematic anatomical screening followed by functional testing, we identified two sleep-promoting neuronal populations along a thalamo-amygdala pathway, both expressing neurotensin (NTS). Rabies-mediated monosynaptic retrograde tracing identified...
Articles
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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