G1N350

Sigma-Aldrich

GenElute Mammalian Genomic DNA Miniprep Kits

sufficient for 350 purifications

Synonym(s):
Mammalian Genomic DNA Miniprep, Gen Elute
NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 350 purifications

storage temp.

room temp

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General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

GenElute Mammalian Genomic DNA Miniprep Kits has been used:
  • to extract DNA from fin samples
  • to extract the BSTUI restriction enzyme from the DNA
  • to isolate DNA from the blood samples extracted from caudal fin
  • to extract tissue and hair follicle DNA

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Features and Benefits

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Principle

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2112Column Preparation Solution

  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

  • R6148RNase A solution

Signal Word

Danger

RIDADR

UN 3316 9

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Inbreeding evidence in a traditional channel catfish (Ictalurus punctatus) hatchery in Mexico
Parra-Bracamonte G M, et al.
Electronic journal of Biotechnology, 14(6), 11-11 (2011)
Gudrun Dittrich-Schröder et al.
Molecular ecology resources, 12(1), 109-115 (2011-09-29)
DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of...
Genetic shifts in the transition from wild to farmed white-tailed deer (Odocoileus virginianus) population
Hernandez-Mendoza P M, et al.
International Journal of Biodiversity Science, Ecosystem Services & Management, 10(1), 3-8 (2014)
Takahiro Seki et al.
Journal of controlled release : official journal of the Controlled Release Society, 156(3), 381-389 (2011-09-03)
Tumour Necrosis Factor alpha (TNF) is a pleiotropic pro-inflammatory cytokine with known vascular permeabilising activity. It is employed during isolated limb perfusion to enhance delivery of chemotherapeutic drugs into tumour tissue. The use of conditionally-replicating lytic viruses, so called 'oncolytic...
Novel single nucleotide polymorphisms in candidate genes for growth in tilapia (Oreochromis niloticus)
Cuevas-Rodriguez B L, et al.
Revista Brasileira de Zootecnia, 45(6), 345-348 (2016)
Protocols
The GenElute Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.
Read More
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
Read More
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Read More
Related Content
The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian cells and tissues. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.
Read More

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