G1N70

Sigma-Aldrich

GenElute Mammalian Genomic DNA Miniprep Kits

sufficient for 70 purifications

Synonym(s):
Mammalian Genomic DNA Miniprep, Gen Elute
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 70 purifications

storage temp.

room temp

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General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Features and Benefits

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Principle

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2112Column Preparation Solution

  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

  • R6148RNase A solution

Signal Word

Danger

Target Organs

Respiratory system

RIDADR

UN 3316 9

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
C M Vaz et al.
Acta biomaterialia, 1(5), 575-582 (2006-05-17)
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Journal of the American Academy of Dermatology, 66(1), 46-50 (2011-06-28)
Human herpesvirus (HHV) 8, an essential etiologic agent of Kaposi sarcoma, is also associated with several lymphoproliferative disorders. The involvement of HHV 8 in mycosis fungoides (MF) and large plaque parapsoriasis (LPP) is controversial, with contradictory reports from various countries...
Inés Sanchez-Roman et al.
Biogerontology, 13(4), 399-411 (2012-05-15)
It is known that a global decrease in food ingestion (dietary restriction, DR) lowers mitochondrial ROS generation (mitROS) and oxidative stress in young immature rats. This seems to be caused by the decreased methionine ingestion of DR animals. This is...
Matthew J Binnington et al.
Pharmacogenetics and genomics, 22(6), 429-440 (2012-05-10)
Alaska Native (AN) people have a high prevalence of tobacco use and associated morbidity and mortality when compared with the general USA population. Variations in the CYP2A6 and CYP2B6 genes, encoding enzymes responsible for nicotine metabolic inactivation and procarcinogen activation...
Michael L Litvack et al.
Molecular immunology, 48(1-3), 37-47 (2010-11-03)
Innate immune collectin surfactant protein D (SP-D) and natural immunoglobulin M (IgM) are two soluble proteins. These opsonic proteins are good candidates for enhancing late apoptotic cell clearance. However, effects of these proteins on late apoptotic cell clearance in the...
Protocols
The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.
Read More
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
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An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Read More
Related Content
The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian cells and tissues. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.
Read More
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Read More

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