G2794

Sigma-Aldrich

GC10 Competent Cells

for generation of cDNA libraries and DNA plasmid production

MDL number:

grade

for molecular biology

form

suspension

availability

available only in USA

shipped in

dry ice

storage temp.

−70°C

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General description

GC10 are competent E. coli with a transformation efficiency of >1x109 cfu/μg when transformed with non-saturating amounts of pUC19 plasmid DNA. They are comparable to DH10β strain.

Application

Suitable for recovery of high quality plasmid DNA and generation of cDNA libraries from plasmid-based vectors
GC10 chemically competent cells are comparable to the popular DH10β strain and carry recA1 and endA1 mutations that aid in plasmid stability and improved quality of prepared plasmid DNA. The GC10 strain allows beta-galactosidase α-complementation for blue/white screening, is ideal for cloning large plasmids and methylated DNA and is T1 bacteriophage resistant.

Features and Benefits

  • Ensures recovery of stable, high quality plasmid DNA as well as methylated DNA
  • Renders protection to clonal stocks from T1 and T5 bacteriophage contamination
  • Allows for β-galactosidase α-complementation for blue/white screening
  • Guaranteed high transformation efficiency
  • Available as 200 μL aliquots

Components

  • GC10 chemically competent cells, 5 X 200 μL (G2544)
  • pUC 19 control DNA (10 ng/μL), 10 μL (D2567)

Principle

GC10 is K strain bacteria that contain mutations in recA1 and endA1 genes. These mutations aid in minimizing recombination and ensuring plasmid stability. This strain also contains tonA genotype that confers resistance to lytic bacteriophages such as T1 and T5. The host restriction systems are eliminated to allow the cloning of methylated DNA.

Other Notes

For more information on competent cell selection, the definition of genotypes and to view a presentation on competent cells, please visit www.sigma-aldrich.com/competentcells.

Legal Information

DH10β is a trademark of Invitrogen Corp.
GC10 is a trademark of GeneChoice, Inc.

RIDADR

NONH for all modes of transport

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Are home-made competent cells as efficient at transformation as purchased cells?

    They can be, depending on the technique used, the expected transformation efficiency and how the cells are handled.  For special applications, competent cells prepared in-house using standard methods may not provide the efficiency you need.

  6. Are all competent cells suitable for both plasmid production and protein expression?

    No.  Most strains of competent cells are suitable for producing plasmid DNA that will be used to transfect insect or mammalian cells for expression of the protein.  The BL21 competent cell strains have special traits enabling protein expression in bacteria.

  7. What are important considerations when performing bacterial transformation?

    Things to consider when planning bacterial transformation:DNA impuritiesSource of DNAAmount of DNA usedStorage and handling of the competent cells

  8. Can I store the competent cells in the -20C?

    No.  Competent cells should not be stored at -20C for any length of time.  The cells suffer a dramatic drop in transformation efficiency when stored higher than -80C.

  9. How should I thaw the competent cells?

    Competent cells should be thawed on ice.  The transformation efficiency is dependent on the proper handling of the cells (maintaining cold temperature until transformation).

  10. Can I re-freeze the vial if I do not use the entire aliquot of competent cells?

    Re-freezing competent cells will result in a decrease in their transformation efficiency.  If the cells are frozen first in a dry ice / ethanol bath before placement in the -80C freezer, the loss will be about two-fold.  If placed directly in the -80C freezer, the loss in transformation efficiency is about five to ten-fold.

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