NONH for all modes of transport
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
At this time, we have used the kit to test only these species. We have received inquiries concerning its use for rat and higher plant tissue, but we have no data at this time.
We have used this kit ony with a UV/Vis spectrophotometer with 1 cm pathlength cuvettes. We have not attempted to modify it for use with a plate reader.
We have not tried using this kit with our CelLytic reagents. We believe it should be suitable for use with C2978 for mammalian cell lysis or C4882 for yeast cell lysis.
We do not recommend the kit for use with whole blood due to interference from hemoglobin.
Here is the procedure as recommended by our Production group (March 2008): Grow S. cerevisiae (we used GP460 private strain) on YEPD medium; harvest cells by centrifugation. Wash the cells [7g] with 20 ml of 20 mM HEPES buffer, pH 7.0. Discard supernatant. Add 1 ml HEPES buffer to make a thick slurry and pour dropwise into liquid nitrogen. Take approx. 2 g of frozen pellet and homogenize with UltraTurrax T-25 while still in liquid nitrogen to make a fine powder. After all the liquid nitrogen has evaporated, take approximately 2 mL of HEPES buffer and add to the 2 g of finely powdered cells. Centrifuge for 5 min in an Eppendorf (benchtop) centrifuge at maximum speed. The supernatant liquid is the source of the enzyme. The total amount of protein received from this procedure was 21 mg (8.8 mg/mL in 2.4 mL total volume). We determined an activity of approximately 0.3 units/mL, using both the UV and DTNB assays.
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