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GW21052

Sigma-Aldrich

Anti-CKLF antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Chemokine-like factor, isoform b

biological source

chicken

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

application(s)

western blot: suitable

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CKLF(51192)

Immunogen

Recombinant chemokine-like factor isoform c
Immunogen Sequence: GI # 7705933, sequence 27-67

Application

Anti-CKLF antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

Chemokine-like factor is a protein encoded by the CKLF gene in humans. It is the first member of the CKLF-like MARVEL transmembrane domain containing member (CMTM) family and its expression is differentially regulated in myoblasts and myotubes. CKLF has a major role in the recruitment and activation of leucocytes that plays an important role in the pathogenesis of allergic diseases. It can induce proliferation and inhibit apoptosis of human vascular smooth muscle cell by down-regulating Bax and Caspase-3 and by the up-regulation of Bcl-2 expression. CKLF1 may play an important role in inflammation and regeneration of skeletal muscle. It may also play an important role in the development of atopic dermatitis, in the process of immune and inflammatory responses of atherogenesis. CKLF1 is a useful marker for regenerating muscle fibers in inflammatory myopathies.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Certificate of Analysis

Certificate of Origin

W Han et al.
The Biochemical journal, 357(Pt 1), 127-135 (2001-06-21)
Cytokines are small proteins that have an essential role in the immune and inflammatory responses. The repertoire of cytokines is becoming diverse and expanding. Here we report the identification and characterization of a novel cytokine designated as chemokine-like factor 1...
Yang Jiao et al.
Zhonghua wai ke za zhi [Chinese journal of surgery], 47(21), 1654-1657 (2010-02-09)
To evaluate the difference of the expression level of chemokine-like factor 1 (CKLF1) mRNA and protein between human atherosclerotic plaques and normal arteries. Real-time quantitative PCR was performed with experimental group (24 cases of atherosclerotic plaques) specimens and control group...
Tao Zhang et al.
Zhonghua yi xue za zhi, 92(29), 2069-2074 (2012-12-21)
To explore the effects of chemokine-like factor 1 (CKLF1) on the growth and apoptosis of human vascular smooth muscle cell and understand its mechanism for the induction of cell apoptosis. After the transfection of human vascular smooth muscle cell with...
Gao-Yun Yang et al.
International journal of biological sciences, 9(8), 759-765 (2013-08-29)
Human chemokine-like factor 1 (CKLF1), a recently discovered chemokine, has a broad spectrum of biological functions in immune-mediated diseases. It is highly expressed on Th2 lymphocytes and is a functional ligand for human CCR4. CKLF1 has a major role in...
Luning Shao et al.
Cellular immunology, 263(2), 188-195 (2010-04-16)
Chemokine-like factor 1 (CKLF1) was the first member of the CKLF-like MARVEL transmembrane domain containing member (CMTM) family to be discovered. Its expression level is increased clearly in peripheral blood lymphocytes upon phytohemagglutinin stimulation, but little is known about the...

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