GW21475

Sigma-Aldrich

Anti-PTPRJ (ab1) antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Receptor-type tyrosine-protein phosphatase η, Anti-CD148
NACRES:
NA.41

biological source

chicken

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

manufacturer/tradename

Genway 15-288-21475

application(s)

western blot: suitable

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PTPRJ(5795)

General description

Protein-tyrosine phosphatase receptor type J (PTPRJ/CD148) is present predominatly in epithelial cells, various tumor cell lines and in lymphocytes. PTPRJ is commonly referred to as CD148 or DEP1.

Immunogen

Immunogen Sequence: GI # 18860900, sequence 206-292
Recombinant protein tyrosine phosphatase, receptor type, J

Biochem/physiol Actions

Protein-tyrosine phosphatase receptor type J (PTPRJ/CD148) is a protein tyrosine phosphatase. It is a negative regulator of T-cell receptor (TCR)-mediated activation of T cells. CD148 affects TCR signaling by interfering with the phosphorylation of phospholipase Cγ1 (PLCγ1) and LAT. CD148 dephosphorylates platelet-derived growth factor β-receptor, resulting in reduction in cell proliferation. CD148 also participates in functioning of cell-cell contacts and adherens junctions. It interacts with p120(ctn), a component of adherens junctions. CD148 associates with receptor tyrosine kinase RET and modulates the tumor phenotype associated with chimeric RET/papillary thyroid carcinoma oncogenes. It dephosphorylates p85 regulatory subunit of phosphoinositide 3-kinase (PI3K) and ERK1/2 (Extracellular signal-regulated kinases). It dephosphorylates and stabilizes epidermal growth factor receptor (EGFR). CD148 negatively regulates Fms-like tyrosine kinase-3 (FLT3) phosphorylation and signaling. It is up-regulated in humans suffering from rheumatoid arthritis and down-regulated in breast tumors. CD148 is a positive regulator of platelet activation and thrombosis.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Nobuo Tsuboi et al.
The Biochemical journal, 413(1), 193-200 (2008-03-20)
CD148 is a transmembrane tyrosine phosphatase that has been implicated in the regulation of cell growth and transformation. However, the signalling mechanisms of CD148 are incompletely understood. To identify the specific intracellular molecules involved in CD148 signalling, we carried out...
Leslie J Holsinger et al.
Oncogene, 21(46), 7067-7076 (2002-10-09)
The receptor-like protein tyrosine phosphatase DEP1, also known as CD148, is expressed predominantly in epithelial cells, in a variety of tumor cell lines, and in lymphocytes. Expression of DEP1 is enhanced at high cell density, and this observation suggests that...
M Kovalenko et al.
The Journal of biological chemistry, 275(21), 16219-16226 (2000-05-24)
Ligand stimulation of PDGF beta-receptors leads to autophosphorylation of the regulatory tyrosine 857 and of tyrosine residues that in their phosphorylated form serve as docking sites for Src homology 2 domain-containing proteins. Regulation of the PDGF beta-receptor by protein-tyrosine phosphatases...
Yotis A Senis et al.
Blood, 113(20), 4942-4954 (2009-02-28)
Platelets play a fundamental role in hemostasis and thrombosis. They are also involved in pathologic conditions resulting from blocked blood vessels, including myocardial infarction and ischemic stroke. Platelet adhesion, activation, and aggregation at sites of vascular injury are regulated by...
Francesca Sacco et al.
The Journal of biological chemistry, 284(33), 22048-22058 (2009-06-06)
Density-enhanced phosphatase-1 (DEP-1) is a trans-membrane receptor protein-tyrosine phosphatase that plays a recognized prominent role as a tumor suppressor. However, the mechanistic details underlying its function are poorly understood because its primary physiological substrate(s) have not been firmly established. To...

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