Hyaluronic acid sodium salt from rooster comb

avian glycosaminoglycan polysaccharide

Poly(β-glucuronic acid-1→3-β-N-acetylglucosamine-1→4), alternating
CAS Number:
MDL number:
PubChem Substance ID:
Pricing and availability is not currently available.

biological source

chicken (rooster comb)



storage temp.




InChI key



Hyaluronic acid sodium salt from rooster comb has been used:
  • as a component of bi-layer gelatin scaffold
  • as a vehicle for release of gentamicin during left ear surgery in guinea pigs
  • in hyaluronic acid binding assay in human embryonic kidney cell line 293T


100, 250 mg in poly bottle
1 g in poly bottle

Biochem/physiol Actions

High molecular mass polymer composed of repeating dimeric units of glucuronic acid and N-acetyl glucosamine which forms the core of complex proteoglycan aggregates found in extracellular matrix.
Hyaluronic acid is a polysaccharide present in the skin, connective tissue, vitreous humor and synovial fluid. It is essential for tissue hydration and functions to regenerate skin post injury or wounds. Hyaluronic acid, apart from having dermatologic and ophthalmologic applications, serves as a drug delivery vehicle. Hyaluronic acid also exhibits antioxidant and antiglycation properties.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of Hyaluronic acid, Product H5388?

    Product No. H5388 is a linear heteropolysaccharide with repeating 3-O-(β-D-glucuronido)-N-acetyl-D-glucosamine units linked by β-(1-->4)) hexosaminidic bonds.  The molecular weight of this subunit has not been determined, but could be calculated from the information above. Molecular weights of hyaluronic acids vary from 0.1-10 million, depending on the source.  The hyaluronic acid from rooster comb is expected to have a molecular weight of 1-4 million.

  6. What is the purity of Hyaluronic acid, Product H5388?

    Sigma does not perform purity testing on this product due to the variable molecular weight and degradation that may occur in native glycosaminoglycans.  This product is tested for activity and protein content which provide information regarding impurities and suitability.

  7. What is the solubility of Hyaluronic acid, Product H5388?

    For Sigma's analytical test, 15 mg of this product is dissolved in 5 mL of 0.3 M phosphate buffer, pH 5.35 at 25°C (i.e., 3 mg/mL).  

  8. My question is not addressed here, how can I contact Technical Service for assistance?

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  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Hyaluronan binding motifs of USP17 and SDS3 exhibit anti-tumor activity
Ramakrishna S, et al.
PLoS ONE, 7(5), e37772-e37772 (2012)
Extraction, purification and characterization of hyaluronic acid from Rooster comb
Kulkarni SS, et al.
Journal of Applied and Natural Science, 10(1), 313-315 (2018)
Hyaluronic acid enhances gene delivery into the cochlea
Shibata SB, et al.
Human Gene Therapy, 23(3), 302-310 (2011)
Effect of ultrasonic degradation of hyaluronic acid extracted from rooster comb on antioxidant and antiglycation activities
Hafsa J, et al.
Pharmaceutical biology, 55(1), 156-163 (2017)
Purification and characterization of hyaluronic acid from chicken combs
Rosa CS, et al.
Ciencia Rural, 42(9), 1682-1687 (2012)
There are five identified glycosaminoglycan chains (see Figure 1): Hyaluronan is not sulfated, but the other glycosaminoglycan chains contain sulfate substituents at various positions of the chain.
Read More
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Read More
This procedure may be used for Hyaluronidase products.
Read More

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