All Photos(4)

HPA002567

Sigma-Aldrich

Anti-ERH antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):
Anti-Enhancer of rudimentary homolog antibody produced in rabbit
Human Protein Atlas Number:

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μL

application(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:200-1:500

immunogen sequence

LVQPTKRPEGRTYADYESVNECMEGVCKMYEEHLKRMNPNSPSITYDISQLFDFIDDLADLSCLVYRADTQTYQPYNKDWIKEKIYVLLRRQAQQAGK

conjugate

unconjugated

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ERH(2079)

General description

ERH (enhancer of rudimentary homolog) also known as DROER. It is a small nuclear protein which forms a homodimer through the β sheet surface. The polypeptide chain of the protein is composed of three α helices situated on one side of a four-stranded β sheet that forms a unique α-β fold. It is expressed in almost all normal adult and fetal human tissues and its expression is upregulated in human breast, ovarian and liver cancers.

Immunogen

Enhancer of rudimentary homolog recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Biochem/physiol Actions

ERH (enhancer of rudimentary homolog) acts as an enhancer of the rudimentary gene in Drosophila melanogaster, in the pyrimidine metabolic pathway. It is also involved with different cellular processes, such as development of the wings, pyrimidine metabolism, cell cycle progression and proliferation, regulation of the Notch signaling pathway, and transcription control. In humans, it is expressed in all normal human tissues. Its function is not clearly reported. It may have an impact on the transcriptional regulation as well as in the mitotic phase of the cell cycle. During cell cycle, it binds to the Sm complex, essential for the mRNA splicing of the mitotic motor protein centromere-associated protein E (CENP-E), a mitotic kinesin. ERH stabilizes the kinetochore-microtubule attachment. It also has high impact on the genomic stability and cancer cell survival.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST70550.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK Germany

WGK 1

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Meng-Tzu Weng et al.
Protein & cell, 4(11), 807-812 (2013-10-01)
Enhancer of rudimentary homolog (ERH) is a small, highly conserved protein among eukaryotes. Since its discovery nearly 20 years ago, its molecular function has remained enigmatic. It has been implicated to play a role in transcriptional regulation and in cell...
Monika I Banko et al.
PloS one, 8(8), e74885-e74885 (2013-09-10)
ERH is a small, highly evolutionarily conserved nuclear protein of unknown function. Its three-dimensional structure is absolutely unique and it can form a homodimer through a β sheet surface. ERH has been shown to interact, among others, with PDIP46/SKAR and...
Akiko Fujimura et al.
Biochemical and biophysical research communications, 423(3), 588-592 (2012-06-19)
The enhancer of rudimentary homolog (ERH) is a small eukaryotic protein that is highly conserved in animals, plants, and protists but not in fungi. ERH has several binding proteins and has been associated with various cellular processes, such as pyrimidine...
M Isomura et al.
Genomics, 32(1), 125-127 (1996-02-15)
We have isolated a novel human cDNA clone that encodes a protein with 80% identity in amino acid sequence to DROER, the enhancer of the rudimentary gene in Drosophila melanogaster. The rudimentary gene product is thought to have significant enzymatic...
Jessie M Sutherland et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(7), 2759-2768 (2015-03-19)
Controlled gene regulation during gamete development is vital for maintaining reproductive potential. During the process of gamete development, male germ cells experience extended periods of inactive transcription despite requirements for continued growth and differentiation. Spermatogenesis therefore provides an ideal model...

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