I1783

Sigma-Aldrich

Anti-phospho-Insulin Receptor (IR) (pTyr972) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... INSRR(3645)
mouse ... Insr(16337)

General description

The biological effects of insulin are mediated by insulin receptor (IR) and IGF-1 receptor. Insulin receptor consists of two α and two β subunits, each subunit has a tyrosine kinase domain. Signaling by IR is important in cell proliferation and metabolism of glucose. Insulin receptor activates the PI3K/Akt and the FKHR pathway, thereby regulates metabolism and insulin-dependent gene expression. Tyrosine 972 is an autophosphorylation site that is present in the juxtamembrane domain. Phosphorylation at Tyr972 is required for interaction of IR with its intracellular substrates and increases the stability of IR and substrate complexes. Overexpression of IR has been observed in breast cancer tissue and is implicated in proliferation of breast cancer cells, hyperinsulinemia and insulin resistance. IR can also bind IGF-I and IGF-II with decreased affinity
Anti-phospho-IR (pTyr972) recognizes human and mouse IR phosphorylated on tyrosine 972. It may cross react with IGF-1R.

Specificity

This antibody may also have cross-reactivity with phosphosphorylated insulin-like growth factor-1 receptor (IGF-1R).

Immunogen

phosphopeptide derived from the region of human insulin receptor (IR) that contains tyrosine 972.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 50% glycerol with 1 mg/ml of bovine serum albumin and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Antonino Belfiore et al.
Endocrine-related cancer, 18(4), R125-R147 (2011-05-25)
The widespread epidemic of obesity and type 2 diabetes has raised concern for the impact of these disorders as risk factors for cancer and has renewed the interest for studies regarding the involvement of hyperinsulinemia and insulin receptor (IR) in...
V Papa et al.
Trends in endocrinology and metabolism: TEM, 8(8), 306-312 (2008-04-15)
It is commonly believed that the insulin receptor mainly mediates the metabolic effects of insulin, whereas the closely related IGF-I receptor is considered a major factor for the regulation of cell proliferation. Experimental and epidemiological evidence indicates, however, that insulin...
V Papa et al.
The Journal of clinical investigation, 86(5), 1503-1510 (1990-11-01)
The growth of breast cancer cells is under the regulation of hormones, growth factors, and their receptors. In the present study, we have employed a new, sensitive, and specific radioimmunoassay for the direct measurement of insulin receptors in surgical specimens...
Y Kido et al.
The Journal of clinical endocrinology and metabolism, 86(3), 972-979 (2001-03-10)
The pleiotropic actions of insulin are mediated by a single receptor tyrosine kinase. Structure/function relationships of the insulin receptor have been conclusively established, and the early steps of insulin signaling are known in some detail. A generally accepted paradigm is...
Shailendra P Singh et al.
Journal of immunology (Baltimore, Md. : 1950), 201(5), 1491-1499 (2018-07-25)
A link between obesity and periodontitis has been suggested because of compromised immune response and chronic inflammation in obese patients. In this study, we evaluated the anti-inflammatory properties of Kavain, an extract from Piper methysticum, on Porphyromonas gingivalis-induced inflammation in...

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