I3911

Sigma-Aldrich

Protease Inhibitor Cocktail, Animal Component Free

for general use, lyophilized powder

NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

form

lyophilized powder

solubility

water: soluble

storage temp.

−20°C

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General description

This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid-proteases, and aminopeptidases.
Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

Animal component free (ACF). Optimized and tested for general use. Protease Inhibitor Cocktail, Animal Component Free has been used to prevent protein lysis in saliva samples, peritoneal residential macrophage cells and in embryo culture medium.

Components

AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Quantity

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Physical form

The components of this ACF cocktail do not originate from animal sources and can thus be used in the production of animal free proteins.
Lyophilized powder that is water soluble.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Codes

C

Risk Statement

34

Safety Statement

26-36/37/39-45

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
null
Early protein profile of human embryonic secretome.
Foresta C, et al.
Frontiers in Bioscience, 21, 620-634 (2016)
Carlo Foresta et al.
Frontiers in bioscience (Landmark edition), 21, 620-634 (2015-12-29)
Embryos obtained by in vitro fertilization are currently assessed by morphology, but displays limitations with over 70% of embryos failing to implant. In this study, we performed HPLC-MS/MS analysis on the conditioned medium obtained from 50 human embryos at the...
Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
Lopucki M, et al.
Molecular Medicine Reports, 17(6), 8253-8259 (2018)
Functional evaluation of multiple human transgenes to overcome immunological and coagulation barriers associated with pig-to-human xenotransplantation.
Bongoni, A.K. et al.
Thesis, 108-108 (2013)

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