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I9767

Sigma-Aldrich

Anti-IKKβ antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-IκB Kinase β, Anti-IKK2
MDL number:
NACRES:
NA.44

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 85 kDa

species reactivity

human

application(s)

western blot: 1:500 using a whole extract of human epitheloid carcinoma HeLa cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CHUK(1147)

General description

IKK β is a component of I-κ-B-kinase (IKK) core complex consisting of IKK α, IKK β and IKK γ and belongs to Ser/Thr protein kinase family. It has a pivotal role in NF-κ-B signaling pathway. Anti-IKK β antibody can be used in immunoblot (diluted 1:1000). Rabbit anti-IKK β antibody reacts specifically with human IKK β (85 kD).

Immunogen

Synthetic peptide located near the C-terminal region of human IKKβ (amino acids 720-735) conjugated to KLH.

Application

Anti-IKK β antibody can be used in western blotting (diluted 1:500) by using whole extract of human epitheloid carcinoma HeLa cell line.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

J A DiDonato et al.
Nature, 388(6642), 548-554 (1997-08-07)
Nuclear transcription factors of the NF-kappaB/Rel family are inhibited by IkappaB proteins, which inactivate NF-kappaB by trapping it in the cell cytoplasm. Phosphorylation of IkappaBs marks them out for destruction, thereby relieving their inhibitory effect on NF-kappaB. A cytokine-activated protein...

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