KCQS05

Sigma-Aldrich

KiCqStart® Probe qPCR ReadyMix, Low ROX

with Low ROX for ABI and Stratagene instruments

Synonym(s):
Kicqstart Probe qPCR ReadyMix
NACRES:
NA.55
Pricing and availability is not currently available.

form

liquid

feature

hotstart

storage condition

protect from light

color

light blue

shipped in

dry ice

storage temp.

−20°C

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General description

KiCqStart Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. KiCqStart Probe qPCR ReadyMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Applied Biosystems ViiA 7
  • Applied BIosystems QuantStudio
  • Agilent AriaMx
  • Douglas Scientific IntelliQube
  • QIAGEN Rotor-Gene Q
  • Stratagene Mx3000P®
  • Stratagene Mx3005P®
  • Stratagene Mx4000

Features and Benefits

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Legal Information

KiCqStart is a registered trademark of Qiagen Beverly Inc.
Mx3000P is a registered trademark of Agilent Technologies, Inc.
Mx3005P is a registered trademark of Agilent Technologies, Inc.
Mx4000 is a trademark of Agilent Technologies, Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Articles
Probe based QPCR utilizes a fluorescent–labeled target-specific probe resulting in increased specificity and sensitivity. Additionally, a variety of fluorescent dyes are available so that multiple primers can be used to simultaneously amplify many sequences.
Read More
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Read More
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Read More
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Read More
Protocols
KiCqStart™ Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments.
Read More

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