The gene for leptin (LEP) is mapped to the human chromosome 7q32.1. The encoded protein is a non-glycosylated polypeptide, primarily produced and secreted by the mature adipocytes. Leptin is expressed in white adipose tissue, fetal tissues, skeletal muscle, chondrocytes, gastric chief cells, the placenta, ovary, and many immune cells. It is absent or expressed at extremely low levels in other adult tissues. Human leptin is 85% identical to mouse leptin and 84% identical to rat leptin, while mouse and rat exhibit 96% identity in their leptin molecules.
Anti-Leptin reacts specifically with leptin.
synthetic peptide corresponds to the amino terminal (amino acid residues 22-40) of human/mouse leptin.
Anti-Leptin antibody produced in rabbit has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism and gastric mobility. The pathways that mediate the effect of leptin are Janus kinase (JAK)/signal transducer and activator of transcription (STAT), phosphoinositide 3-kinase (PI3K) and c-Jun N-terminal kinase (JNK).
Leptin is a circulating cytokine that induces pleiotropic effects on nutrient intake, thermogenesis, metabolism, reproduction and hematopoiesis. Leptin is hypothesized to be a “satiety factor” because the absence of this factor is associated with hyperphagia and obesity in ob/ob mice (homozygous for the obese spontaneous mutation). It appears that leptin is involved in appetite regulation since leptin injection into ob/ob mice reduces their food intake and ultimately their body weight. However, studies have demonstrated that its effects must be more complicated than simple appetite suppression.
Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.
The antibody is affinity isolated on peptide-agarose.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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